Responses of antioxidant enzyme and photosynthesis in rape seedling to the combined stresses of acid rain and ultraviolet-B radiation

Article type: Research Article

Authors: LIANG, Chan-juan | HUANG, Xiao-hua | TAO, Wen-yi | ZHOU, Qing^;

Affiliations: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, China | College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China | School of Biotechnology,Southern Yangtze University, Wuxi 214036, China

Note: [] Corresponding author. E-mail: zhouqeco@yahoo.com.cn

Abstract: Effects of the simulated acid rain (AR) and ultraviolet-B (UV-B, 280–320 nm) radiation with a single or two ways simultaneously (AR + UV-B) on the antioxidant enzyme and photosynthesis of the rape seedlings were investigated by the hydroponic culture. The results of static experiment indicated that the tolerance of rape seedling to single stress(AR or UV-B) is stronger than that to dual stresses (AR+UV-B). Furthermore, the dual stresses had additive effect on catalase activity, and a synergistic effect on MDA content, net photosynthesis rate, water use efficiency as well as intercellular CO2 concentration. Meanwhile, it has an independent effect on chlorophyll content, stomatal conductance, and transpiration rate as well as membrane permeability. During 64 h restoration course, the dynamic change in the curves of physiological and biochemical indices were not identical, and none of them show a simple linear variation. According to the static and dynamic experiments, it was found that a responsive sequence of catalase activity, membrane permeability, MDA content and photosynthetic characteristics to the above-mentioned stresses was as follows: AR + UV-B > UV-B > AR.

Keywords: combined stresses, elevated UV-B radiation, simulated acid rain, rape seedling, CAT activity, photosynthesis

Journal: Journal of Environmental Sciences, vol. 17, no. 6, pp. 1038-1041, 2005