Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay

Article type: Research Article

Authors: YU, Shui-li | LIU, Ya-nan | JING, Guo-lin | ZHAO, Bing-jie | GUO, Si-yuan

Affiliations: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China

Note: [] Corresponding author. E-mail: liuyanan@gmail.com

Abstract: To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and γ-protebacteria only exited in 1 # reactor. The microbiological diversity of 1 # reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria (AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.

Keywords: 16S rDNA, carbon source, biological phosphorus removal, PCR-DGGE

Journal: Journal of Environmental Sciences, vol. 17, no. 4, pp. 611-614, 2005