Effects of salinity on activities of H^+-ATPase, H^+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean (Glycine max L.) seedlings

Article type: Research Article

Authors: YU, Bing-jun^; | LAM, Hon-ming | SHAO, Gui-hua^; | LIU, You-ling

Affiliations: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China | Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Note: [] Corresponding author. E-mail: bjyu@njau.edu.cn

Abstract: The effects of NaCl stress on the H^+-ATPase, H^+-PPase activity and lipid composition of plasma membrane (PM) and tonoplast (TP) vesicles isolated from roots and leaves of two soybean cultivars (Glycine max L.) differing in salt tolerance (Wenfeng7, salt-tolerant; Union, salt-sensitive) were investigated. When Wenfeng7 was treated with 0.3% (W/V) NaCl for 3 d, the H^+-ATPase activities in PM and TP from roots and leaves exhibited a reduction and an enhancement, respectively. The H^+-PPase activity in TP from roots also increased. Similar effects were not observed in roots of Union. In addition, the increases of phospholipid content and ratios ofphospholipid to galactolipid in PM and TP from roots and leaves of Wenfeng7 may also change membrane permeability and hence affect salt tolerance.

Keywords: salinity, Glycine max L., plasma membrane, tonoplast, H[TeX:] ^+-ATPase, H-P[TeX:] ^+Pase, membrane lipid composition

Journal: Journal of Environmental Sciences, vol. 17, no. 2, pp. 259-262, 2005