Effects of β-cyclodextrins on the enzymatical hydrolysis of chiral dichlorprop methyl ester

Article type: Research Article

Authors: WEN, Yue-zhong | ZHOU, Shan-shan | FANG, Zhao-hua | LIU, Wei-ping

Affiliations: Institute of Environment Science, Zhejiang University, Hangzhou 310029, China

Note: [] Corresponding author. E-mail: wenyuezhong@zjuem.zju.edu.cn

Abstract: The effect of β-cyclodextrins (β-CDs) on the enzymatical hydrolysis of chiral dichlorprop methyl ester (DCPPM) was studied.Four kinds of β-cyclodextrins (β-cyclodextrin, Partly methylated-CD (PM-β-CD), hydroxypropyl-cyclodextrin (HP-β-CD) and carboxymethyl-cyclodextrin (CM-β-CD) ) were used. Compared with 100% DCPPM in the absence of β-cyclodextrins, the activity of lipase decreased with the increase of β-cyclodextrin and PM-β-cyclodextrin. However, CM-β-cyclodextrin stimulated the lipase activity. The inhibition effect of β-cyclodextrin and PM-β-cyclodextrin on the hydrolysis of DCPPM is affected by many factors other than degree of the methylation blocking the active site of lipase. UV-Vis and Fourier transform infrared (FTIR) spectroscopy studies of the complexation of aqueous DCPPM with β-CDs provide fresh insight into the molecular structure of the complex and explain the effects of β-CDs on enzymatical hydrolysis of chiral DCPPM. Data showed that inclusion complexes had formed by complexation of the CM-β-CD with DCPPM and the solubility of DCPPM was increased in water, which leaded to the increased lipase activity.

Keywords: β-cyclodextrins, enzyme, hydrolysis, chiral, dichlorprop

Journal: Journal of Environmental Sciences, vol. 17, no. 2, pp. 237-240, 2005