DNA damage of germ cell of rat induced by nitrotoluene chemicals

Article type: Research Article

Authors: YANG, Li^; | XU, Jing-bo | ZHEN, Lin | SUN, Zhi-wei | SHI, Long | JIN, Ming-hua | LIU, Xiao-mei

Affiliations: Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China | Department of Environmental Science, Northeast Normal University, Changchun 130024, China | School of Public Health, Jilin University, Changchun 130021, China | Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

Note: [] Corresponding author. E-mail: yangl@igsnrr.ac.cn

Abstract: 2,4-dinitrotoluene (2,4-DNT), 2,6-dinitrotoluene (2,6-DNT) and 4-nitrotoluene (4-NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis (SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single-strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4-DNT, 2,6-DNT and 4-NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.

Keywords: nitrotoluenes, sertoli/germ cell cocultures, SCGE, genotoxicity

Journal: Journal of Environmental Sciences, vol. 17, no. 1, pp. 84-90, 2005