Effects of traffic pollution on the genetic structure of Poa annua L. populations

Article type: Research Article

Authors: LI, Ning | CHEN, Xiao-yong^; | SHEN, Lang | LI, Yuan-yuan | CAI, Yue-wei

Affiliations: Department of Environmental Sciences, East China Normal University, Shanghai 200062, China | Key Laboratory of Geographic Information Science, The Ministry of Education, Shanghai 200062, China

Note: [] Corresponding author. E-mail: xychen@des.ecnu.edu.cn

Abstract: The genetic composition of Poa annua L. populations with a series of traffic pollution was studied by starch electrophoresis. Five enzyme systems were stained. The results showed that: (1) Traffic pollution can dramatically change genotypic frequencies at some loci of P. annua populations. Significant deviations from Hardy-Weinberg equilibrium were observed on loci Fe-1 and Me due to the excess of heterozygote in some populations. (2) The effective number of alleles per locus, and the observed and expected heterozygosity were higher in the pollution series than in the clear control site (Botanic Park population), but the increase was not related with the pollution extent. (3) Most genetic variation was found within populations, and only 6.21% was among populations of the polluted series. Slightly higher differentiation (F_{ST}=7.98%) was observed when the control population was included. (4) The calculated gene flow (Nm) is 2.8841 per generation. The mean of genetic identity is 0.9864 and the genetic distance average to 0.0138.

Keywords: genetic composition, traffic pollution, allozyme analysis, Poa annua L

Journal: Journal of Environmental Sciences, vol. 16, no. 3, pp. 454-457, 2004