Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Ordóñez, José L.a; b | Pérez, Rodrigoa | Barriga, Andrésc | Seguel, Ivettea | Guzman, Paolaa | Zúñiga, María C.b | Delporte, Carlaa; *
Affiliations: [a] Laboratorio de Productos Naturales, Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Dr. Carlos Lorca Tobar 964, Santiago, Chile | [b] Laboratorio de Química Inorgánica y Analítica, Departamento de Química inorgánica y Analítica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Dr. Carlos Lorca Tobar 964, Santiago, Chile | [c] Unidad de Espectrometria demasas, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Dr. Carlos Lorca Tobar 964, Santiago, Chile
Correspondence: [*] Corresponding author: Carla Delporte, Laboratorio de Productos Naturales, Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, PO Box 8380492, Dr. Carlos Lorca Tobar 964, Santiago, Chile. E-mail: cdelpor@uchile.cl.
Abstract: BACKGROUND:Sustained hyperglycemia leads to multiple health complications associated with oxidative balance and metabolic pathways alterations. Current treatments for hyperglycemia are not entirely effective thus, the identification of natural products from food sources, such as Ugni molinae berries, to reduce hyperglycemia and prevent the deleterious effect of oxidative stress is attractive to develop new therapeutics. OBJECTIVE:Our aim was to evaluate the antioxidant capacity and the inhibitory activity on α-glucosidase and glycogen phosphorylase A of polyphenolic extracts from different genotypes of U. molinae berries and to comparatively analyze their polyphenolic profile. METHODS:Berry extraction was performed by exhaustive maceration with increasing-polarity solvents. The antioxidant capacity, and inhibitory activity on enzymes were analyzed by different spectrophotometric methods. Moreover, the chemical profile of bioactive extracts was comparatively evaluated through LC-MS. RESULTS:16 semi-purified extracts were obtained and showed antioxidant capacity and inhibitory activity on the evaluated enzymes. Moreover, the chemical analysis showed differences in phenolic profile among the extracts and, particularly, the acetonic extracts were more concentrated in phenolic compounds, which is associated with the more potent activities. CONCLUSIONS:Our results indicate that murtilla berries’ acetonic extracts include a mixture of phenolic compounds that inhibit the activity of two enzymes related to carbohydrate metabolism and have a promising antioxidant capacity.
Keywords: Ugni molinae, berries, antioxidant, α-glucosidase, glycogen phosphorylase A
DOI: 10.3233/JBR-211552
Journal: Journal of Berry Research, vol. 12, no. 2, pp. 279-296, 2022
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl