Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Quesada, María S.a; b; * | Azofeifa, Gabrielaa | Campone, Lucac | Pagano, Immad | Pérez, Ana M.e | Cortés, Carolinae | Rastrelli, Lucad | Quesada, Silviaa
Affiliations: [a] Biochemistry Department, School of Medicine, University of Costa Rica, San José, Costa Rica | [b] Pharmacy Faculty, University of Costa Rica, San José, Costa Rica | [c] Department of Agriculture, Food Chemistry, University Mediterranea of Reggio Calabria, Reggio Calabria, Italy | [d] Pharmacy Department, University of Salerno, Fisciano, Italy | [e] National Center of Food Science and Technology (CITA), University of Costa Rica, San José, Costa Rica
Correspondence: [*] Corresponding author: María S. Quesada, Facultad de Farmacia, Universidad de Costa Rica, Campus Rodrigo Facio, 2060, San José, Costa Rica. Tel.: +506 2511 8345; E-mail: maria.quesada@ucr.ac.cr.
Abstract: BACKGROUND:Lower risk of digestive tract cancer development has been associated with polyphenol intake. Bactris guineensis is an edible endemic palm that grows in Central and South America. OBJECTIVE:This study performs a phenolic characterization of Bactris guineensis and evaluates the bioactivity of this fruit. METHODS:The phenolic compounds of B. guineensis were characterized by HPLC-UV-HRMS analyses and the antioxidant activity was measured by chemical and cellular methods. Additionally, cytotoxicity of B. guineensis polyphenols was performed on 4 cancer cell lines and the pro-apoptotic effect was evaluated by flow cytometry using annexin staining. RESULTS:The major phenolic compounds of B. guineensis were proanthocyanidins. The extract IC50 for DPPH was 3.3±0.2 μg/mL and for induced intracellular ROS was 153±13 μg/mL. MTT cytotoxic assays demonstrate IC50 values between 16.6 and 24.9 μg/mL for the colon and hepatic adenocarcinomas, with high selectivity effects towards cancer cells compared to non-tumor cells. A 20 to 50% early apoptotic effect was observed in cancer cells lines by Annexin/PI staining. CONCLUSIONS:B. guineensis evidenced an important radical scavenging activity and a strong cytotoxic activity against hepatic and colorectal carcinoma cells, showing better values than procyanidin extracts from other fruits previously described.
Keywords: Bactris guineensis , antioxidant, polyphenols, apoptosis, cytotoxicity, procyanidins
DOI: 10.3233/JBR190449
Journal: Journal of Berry Research, vol. 10, no. 3, pp. 329-344, 2020
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl