Examination of the Effect of Rare Variants in TREM2, ABI3, and PLCG2 in LOAD Through Multiple Phenotypes

Article type: Research Article

Authors: Olive, Claudia^{a; b} | Ibanez, Laura^{a; b} | Farias, Fabiana H. Geraldo^{a; b} | Wang, Fengxian^{a; b} | Budde, John P.^{a; b} | Norton, Joanne B.^{a; b; c} | Gentsch, Jen^{a; b; c} | Morris, John C.^{b; c} | Li, Zeran^{a; b} | Dube, Umber^{a; b} | Del-Aguila, Jorge^{a; b} | Bergmann, Kristy^{a; b} | Bradley, Joseph^{a; b} | Benitez, Bruno A.^{a; b} | Harari, Oscar^{a; b} | Fagan, Anne^d | Ances, Beau^d | Cruchaga, Carlos^{a; b; 1} | Fernandez, Maria Victoria^{a; b; 1; *}

Affiliations: [a] Neurogenomics and Informatics Center, Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA | [b] Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MO, USA | [c] Knight Alzheimer’s Disease Research Center, Washington University School of Medicine, St. Louis, MO, USA | [d] Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA

Correspondence: [*] Maria Victoria Fernandez, Neurogenetics and Informatics, Department of Psychiatry, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA. E-mail: fernandezv@wustl.edu.

Note: [1] These authors contributed equally to this work.

Abstract: Background:Rare variants in PLCG2 (p.P522R), ABI3 (p.S209F), and TREM2 (p.R47H, p.R62H) have been associated with late onset Alzheimer’s disease (LOAD) risk in Caucasians. After the initial report, several studies have found positive results in cohorts of different ethnic background and with different phenotype. Objective:In this study, we aim to evaluate the association of rare coding variants in PLCG2, ABI3, and TREM2 with LOAD risk and their effect at different time points of the disease. Methods:We used a European American cohort to assess the association of the variants prior onset (using CSF Aβ42, tau, and pTau levels, and amyloid imaging as endophenotypes) and after onset (measured as rate of memory decline). Results:We confirm the association with LOAD risk of TREM2 p.R47H, p.R62H and ABI3 p.S209F variants, and the protective effect of PLCG2 p.P522R. In addition, ABI3 and TREM2 gene-sets showed significant association with LOAD risk. TREM2 p.R47H and PLCG2 p.P522R variants were also statistically associated with increase of amyloid imaging and AD progression, respectively. We did not observe any association of ABI3 p.S209F with any of the other AD endophenotypes. Conclusion:The results of this study highlight the importance of including biomarkers and alternative phenotypes to better understand the role of novel candidate genes with the disease.

Keywords: ABI3 , endophenotypes, late onset Alzheimer’s disease, PLCG2 , progression, TREM2

DOI: 10.3233/JAD-200019

Journal: Journal of Alzheimer's Disease, vol. 77, no. 4, pp. 1469-1482, 2020