TSPO Ligands Boost Mitochondrial Function and Pregnenolone Synthesis
Abstract
Translocator protein 18 kDa (TSPO) is located in the mitochondrial outer membrane and plays an important role in steroidogenesis and cell survival. In the central nervous system (CNS), its expression is upregulated in neuropathologies such as Alzheimer’s disease (AD). Previously, we demonstrated that two new TSPO ligands based on an imidazoquinazolinone termed 2a and 2b, stimulated pregnenolone synthesis and ATP production in vitro. In the present study, we compared their effects to those of TSPO ligands described in the literature (XBD173, SSR-180,575, and Ro5-4864) by profiling the mitochondrial bioenergetic phenotype before and after treatment and investigating the protective effects of these ligands after oxidative injury in a cellular model of AD overexpressing amyloid-β (Aβ). Of note, ATP levels increased with rising pregnenolone levels suggesting that the energetic performance of mitochondria is linked to an increased production of this neurosteroid via TSPO modulation. Our results further demonstrate that the TSPO ligands 2a and 2b exerted neuroprotective effects by improving mitochondrial respiration, reducing reactive oxygen species and thereby decreasing oxidative stress-induced cell death as well as lowering Aβ levels. The compounds 2a and 2b show similar or even better functional effects than those obtained with the reference TSPO ligands XBD173 and SSR-180.575. These findings indicate that the new TSPO ligands modulate mitochondrial bioenergetic phenotype and protect against oxidative injury probably through the de novo synthesis of neurosteroids, suggesting that these compounds could be potential new therapeutic tools for the treatment of neurodegenerative disease.
INTRODUCTION
Translocator protein 18 kDa (TSPO) is known to facilitate the transport of cholesterol from cytosol to the mitochondrial matrix where it is metabolized into pregnenolone by the cytochrome P450scc [1, 2]. Pregnenolone is the main precursor of the steroid hormones and neurosteroid biosynthesis [3]. TSPO activation can increase steroid synthesis and inhibits apoptosis and inflammation, thereby decreasing cell damage and promoting cell survival [4]. Neuroactive steroids can bind intracellular steroid hormone receptors and act, like traditional steroids, as transcription factors regulating the gene expression. They can also interact with numerous neurotransmitter receptors (e.g., glutamate, gamma-aminobutyric acid (GABA), acetylcholine, norepinephrine, dopamine, and 5-hydroxytryptamine) and regulate neuronal activity [5]. Depending on their pre- or post-synaptic action, they can modulate the synaptic plasticity in specific brain areas and modulate learning, memory, emotion, motivation, and cognition processes [5]. Many central nervous system (CNS) diseases present an upregulation of TSPO including Alzheimer’s disease (AD) in which positron emission tomography on brains of AD patients confirmed an increase of TSPO [6]. The most important endogenous ligands of TSPO are cholesterol and porphyrins showing nanomolar and micromolar affinity for TSPO, respectively [7, 8]. Endozepines, other endogenous ligands of TSPO, are a family of neuropeptides which derives from a polypeptide precursor, the diazepam-binding inhibitor (DBI) that allosterically modulates GABAergic transmission in various neurodegenerative diseases [9]. Biologically active peptide fragments of DBI have been shown to stimulate the mitochondrial steroid synthesis [10]. In cerebrospinal fluid of AD patients, elevated levels of endozepines were measured [11]. Amyloid-β (Aβ), a peptide known to have a key pathogenic role in AD, stimulates the synthesis of endozepines by astrocytes [12]. 4’Chlorodiazepam, a TSPO ligand (LTSPO), has been shown to exert neuroprotective effects against Aβ by a mechanism involving the regulation of apoptosis regulator (e.g., Bax) and surviving factor (e.g., surviving) expression [13].
TSPO ligands could also be effective in neuroprotection by modulating endogenous production of neurosteroids in the nervous system [1, 2, 14]. The stimulation of the neurosteroid synthesis may be a beneficial strategy for AD pathology showing a drop of neurosteroid levels such as allopregnanolone levels in the cerebral cortex of the brain from a triple transgenic mouse model of AD (3xTgAD) as well as postmortem in the brains of humans affected with AD [15, 16]. Thus, neurosteroids can regulate both regeneration and repair mechanisms in the brain and studies showed the ability of allopregnanolone (APα) to promote the regenerative processes in both central and peripheral nervous system [16–21].
In the literature, synthetic ligands of TSPO have been developed for three purposes: 1) to improve the understanding of the underlying molecular mechanisms of TSPO; 2) to be used in medical imaging as markers of inflammation; and 3) to discover new ways to treat diseases affecting the CNS [6]. During the last decades, TSPO ligands were found to increase the level of neurosteroids as pregnenolone and allopregnanolone and therefore studied for their neuroprotective and anxiolytic properties [1, 22–25]. TSPO ligands seem to offer alternative therapeutic strategies focused on reducing the accumulation of Aβ as they simultaneously target multiple facets of the neurodegenerative cascade such as neuroinflammation, oxidative stress, mitochondrial dysfunction, and neuronal loss.
Notably, many TSPO ligands were already described in the literature but suffer from a common problem of solubility. In a previous study from our group, several ligands of TSPO based on an imidazo[1,2-c]quinazolinone scaffold were described with nanomolar affinity and a good selectivity against the central benzodiazepine receptor (see compounds structures and results of binding assay in Supplementary Figures 1 and 2) [26]. In particular, the compounds termed 2a and 2b have been shown to ameliorate the adenosine triphosphate (ATP) production and the production of pregnenolone in human neuroblastoma cells expressing the human amyloid-β protein precursor (AβPP), a cellular model of AD.
TSPO is located in the outer mitochondrial membrane [27–29] and interacts with ligands to modulate various molecular biological mechanisms such as mitochondrial reactive oxygen species (ROS) generation, mitochondria membrane potential (MMP), and ATP production as well as the modulation of nuclear gene expression via mitochondrial-nuclear signaling [28–32]. Mitochondria are placed at the center of this study to investigate the effect of our TSPO ligands because these paramount organelles are not only the main producers of energy in the cells, but also to main source of ROS and the seat of neurosteroidogenesis with the synthesis of pregnenolone.
Besides, mitochondrial dysfunction plays a crucial role in AD pathogenesis and may be placed in the center of the degenerative events. Indeed, the “Alzheimer mitochondrial cascade hypothesis” stated by Swerdlow and Khan (2004) postulates that mitochondrial dysfunction is an early event of the disease which may affect AβPP expression and processing leading to Aβ accumulation [33].
Therefore, based on preliminary findings [26], we aimed to evaluate the effects of the new TSPO ligands compounds 2a and 2b on mitochondrial bioenergetic phenotype, and to test whether they are able to 1) alleviate bioenergetics deficits observed in AβPP/Aβ overexpressing neuroblastoma cells (APP cells), by activating the metabolic activity and ameliorating the mitochondrial respiration; 2) reduce production of Aβ40; and 3) exert protective effects on the APP cells by ameliorating bioenergetics and reducing oxidative injury under stress condition. The effectiveness of the new TSPO ligands 2a and 2b were compared to TSPO ligands described in the literature: XBD173, SSR-180,575, and Ro5-4864 (see structures in Supplementary Figure 1).
MATERIALS AND METHODS
Chemicals and reagents
Dulbecco’s-modified Eagle’s medium (DMEM), fetal calf serum (FCS), penicillin/streptomycin, dihydrorhodamine 123 (DHR123), 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA), adenosine diphosphate (ADP), hydrogen peroxide (H2O2), pyruvate, succinate, and malate were from Sigma-Aldrich (St. Louis, MO, USA). Glutamax and MitoSOX were from Gibco Invitrogen (Waltham, MA, USA). Horse serum (HS) was from Amimed, Bioconcept (Allschwil, Switzerland). Ligand of the receptor TSPO called LTSPO were synthetized as described previously [26] by the laboratory of CNRS, University of Strasbourg, UMR 7200, Faculty of Pharmacology (Strasbourg, France).
Cell culture
Human SH-SY5Y neuroblastoma and human embryonic kidney cells (HEK293) were grown at 37°C in a humidified incubator chamber under an atmosphere of 7.5% CO2 in DMEM supplemented with 10% (v/v) heat-inactivated FCS, 2 mM Glutamax, and 1% (v/v) penicillin/streptomycin. Cells were passaged 1–2 times per week, and plated for treatment when they reached 80–90% confluence. SH-SY5Y cells were stably transfected with DNA constructs harboring human wild-type APP695 (APP) or the expression vector pCEP4 (Invitrogen, Saint Aubin, France) alone (control vector, Co) [34]. Transfected APP cells were grown in DMEM standard medium supplemented with 300 μg/ml hygromycin. HEK cells overexpressing Swedish APP (HEK SWE APP) cells were stably transfected with DNA constructs harboring human wild-type APP695 (APP) and were grown in DMEM standard medium supplemented with 300 μg/ml G418.
Treatment paradigm
On the basis of our previous study, the concentration of 10 nM of LTSPO was selected and used in all assays. SH-SY5Y cells were treated in DMEM+10% FCS one day after plating either with DMEM alone (untreated control condition) or with a final concentration of 10 nM of XBD173, SSR180575, Ro5-4864, 2a, and 2b, made from a stock solution in dimethyl sulfoxide (DMSO), for 24 h (final concentration of DMSO <0.002%, no effect of the vehicle solution (DMSO) alone compared to the untreated condition). For the stress experiments, cells were first pre-treated for 24 h with LTSPOs and then treated for 3 h with 500 μM H2O2. Then ATP assays, mitochondrial respiration (RCR), ROS detection and MTT assays were performed. Each assay was repeated at least 3 times.
ATP levels
Total ATP content of SH-SY5Y cells was determined using a bioluminescence assay (ViaLighTM HT, Cambrex Bio Science, Walkersville, MD, USA) according to the instruction of the manufacturer, as previously described [35, 36]. SH-SY5Y cells were plated in 5 replicates into a white 96-well cell culture plate at a density of 2x104 cells/well. The bioluminescent method measures the formation of light from ATP and luciferin by luciferase. The emitted light was linearly related to the ATP concentration and was measured using the multilabel plate reader VictorX5 (Perkin Elmer).
Pregnenolone direct ELISA
The evaluation of the production of pregnenolone was performed with a direct enzyme-linked immunosorbent assay (ELISA) test (DRG diagnostics ©, Germany), an enzyme immunoassay for the quantitative determination of pregnenolone in Co and APP cells. SH-SY5Y cells were plated in 4–8 replicates into a white 96-well cell culture plate at a density of 2x104 cells/ well overnight. Cells were washed with a saline buffer (140 mM NaCl, 5 mM KCl, 1,8 mM CaCl2, 1 mM MgSO4+7H2O, 10 mM glucose, 10 mM HEPES/NaOH, 0.1% BSA, pH 7.4) and treated with molecules of references or TSPO ligands (20 μM), and incubated for 2 h. In order to measure the production of pregnenolone, the downstream conversion of pregnenolone was blocked by the addition of trilostane (25 μM) and abiraterone (0.1 μM). The cell supernatant was then harvested and the ELISA test was performed according to the manufacture instructions. The plate was read at 450 nm using the plate reader Cytation 3 (Biotek).
Determination of mitochondrial membrane potential
The MMP was measured using the fluorescent dye tetramethylrhodamine, methyl ester, and perchlorate (TMRM). SH-SY5Y cells were plated in 8 replicates into a black 96-well cell culture plate at a density of 2×104 cells/well. Cells were loaded with the dye at a concentration of 0.4 μM for 15 min. After washing twice with HBSS, the fluorescence was detected using the multilabel plate reader VictorX5 (PerkinElmer) at 530 nm (excitation)/590 nm (emission). Transmembrane distribution of the dye was dependent on MMP.
Mitochondrial respiration
The investigation of mitochondrial respiration was performed using the Seahorse Bioscience XF24 Analyzer. XF24 cell culture microplates were coated with 0.1% gelatine and cells were plated at a density of 2.5x104 cells/well in 100 μl of treatment medium containing 10% FCS, 1 g/l glucose, and 4 mM pyruvate. After 24 h of treatment with molecules of references or LTSPO, cells were washed with 1x pre-warmed mitochondrial assay solution (MAS; 70 mM sucrose, 220 mM mannitol, 10 mM KH2PO, 4.5 mM MgCl2, 2 mM HEPES, 1 mM EGTA, and 0.2% (w/v) fatty acid-free BSA, pH 7.2 at 37°C) and 500 μl of pre-warmed (37°C) MAS containing 1 nM XF plasma membrane permeabilizer (PMP, Seahorse Bioscience), 10 mM pyruvate, 10 mM succinate, and 2 mM malate was added to the wells. The PMP was used to permeabilize intact cells in culture, which circumvents the need for isolation of intact mitochondria and allows the investigation of the oxygen consumption rate (OCR) under different respiratory states induced by the sequential injection of: 1) ADP (4 mM) to induce state 3; 2) oligomycin (0.5 μM) to induce state 4o; Data were extracted from the Seahorse XF24 software and the RCR (state 3/state 4o), which reflects the mitochondrial respiratory capacity, was calculated.
Oxygen consumption rate and extracellular acidification rate
The Seahorse Bioscience XF24 Analyzer was used to perform a simultaneous real-time measurement of OCR and extracellular acidification rate (ECAR). XF24 cell culture microplates (Seahorse Bioscience) were coated with 0.1% gelatine and SH-SY5Y cells were plated at a density of 2.5x104 cells / well in 100 μl of the treatment medium containing 10% FCS, 1 g/l glucose, and 4 mM pyruvate. After 24 h of treatment with molecules of references or LTSPO treatment, cells were washed with PBS and incubated with 500 μl of assay medium (DMEM, without NaHCO3, without phenol red, with 1 g/l glucose, 4 mM pyruvate, and 1% L-glutamine, pH 7.4) at 37°C in a CO2-free incubator for 1 h. The plate was placed in the XF24 Analyzer and basal OCR and ECAR were recorded during 30 min.
MTT assays
To assess cell viability, MTT reduction assays were performed according to the manufacturer’s protocol (Cell proliferation kit I (MTT), Roche, Germany). Briefly, native and genetically modified SH-SY5Y cells were seeded at 2x104 cells / well into 96-well plates and allowed to attach. After 24 h, neuroblastoma cells were incubated under the following conditions:
To evaluate the protective effects of the selected TSPO ligands 2a and 2b, cells were pre-treated for 24 h at a concentration of 10 nM and then incubated for 3 h with a concentration of 500 μM of H2O2 capable of killing about 70% of genetically modified SH-SY5Y cells (APP cells). Values were normalized to the control groups treated with H2O2 alone.
Detection of Aβ levels
The Human Aβ40 ELISA kit was used for the quantitative determination of human Aβ40 in cell culture supernatants. The ELISA was performed in accordance with the Aβ-ELISA kit by Invitrogen. The assay principle is that of a monoclonal antibody specific for the NH2-terminus of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known human Aβ40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1–40 Aβ sequence. This COOH-terminal sequence is created upon cleavage of the analyzed precursor. After washing, bound rabbit antibody is detected by the addition of a horseradish peroxidase–labeled anti-rabbit antibody. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human Aβ40 present in the original specimen.
Reactive oxygen species detection
Levels of cytosolic ROS, mitochondrial reactive oxygen species, and specific levels of mitochondrial superoxide anion radicals were assessed using the fluorescent dyes H2DCF-DA, DHR123 and the Red Mitochondrial Superoxide Indicator (MitoSOX), respectively. SH-SY5Y cells were plated in 6 replicates into a black 96-well cell culture plate at a density of 2x104 cells/well. After LTSPO treatment, cells were loaded with 10 μM of DCF or DHR for 15 min or 5 μM of MitoSOX for 90 min at room temperature in the dark on an orbital shaker. After washing twice with HBSS (Sigma), the formation of green fluorescent products, DCF and DHR, generated by the oxidation of H2DCF-DA and DHR123, respectively, were detected using the multilabel plate reader VictorX5 at 485 nm (excitation)/538 nm (emission). MitoSOX, which is specifically oxidized by mitochondrial superoxide, exhibits a red fluorescence detected at 535 nm (excitation)/595 nm (emission). The intensity of fluorescence was proportional to mitochondrial ROS levels, cytosolic ROS level and superoxide anion radicals in mitochondria.
Statistical analysis
Data are given as the mean±SEM, normalized to the untreated control group (=100%). Statistical analyses were performed using the Graph Pad Prism software. For statistical comparisons of more than two groups, One-way ANOVA was used, followed by Dunnett’s multiple comparison tests versus the control. For statistical comparisons of two groups, Student unpaired t-test was used. The experimental data are evaluated using the GraphPad-Prism program (GraphPad-Prism, San Diego, CA, USA). p-values <0.05 were considered statistically significant. The goodness of fits was estimated by the R-squared value (>0.9) using Pearson correlation and linear regression analysis.
RESULTS
TSPO ligands increased bioenergetics
After a treatment with the TSPO ligands (24 h, at 10 nM), a significant increase in pregnenolone (PREG), ATP, and MMP levels was detected for the ligands in Co cells (2a.: PREG: +25.7%, ATP: +23.4%, MMP: +30%; 2b: PREG: +24.9%, ATP: +20.10%, MMP: +16.3%) and in APP cells (2a: PREG: +65.10%, ATP: +15.5%, MMP: +29.4%; 2b: PREG: +62.8%, ATP: +16.4%, MMP: +24.5%) when compared to untreated cells (Fig. 1) confirming our preliminary findings [26]. We then next assessed whether the TSPO ligand-induced increase of pregnenolone levels correlated with ATP or MMP levels after treatment (24 h, at 10 nM) in Co cells (Fig. 1A, C) and APP cells (Fig. 1B, D) [26]. For that purpose, Pearson correlations were performed. XDB173 and SSR-180,575, but not Ro5-4864, had significant effects on ATP, MMP, and pregnenolone levels similar to those of 2a and 2b. Notably, significant positive linear correlations were found between ATP and pregnenolone levels in Co cells (Fig. 1A, p = 0.0188, R = 0.8750) and APP cells (Fig. 1B, p = 0.0103, R = 0.9174) after treatment with the TSPO ligands. In addition, MMP and pregnenolone levels significantly correlated in Co cells (Fig. 1 C, p = 0.0155, R = 0.8923) as well as APP cells (Fig. 1D, p = 0.0302, R = 0.8343). These data suggest that the raise in ATP and MMP levels were preferentially linked to an increase of pregnenolone level.
Fig.1
Significant correlations between ATP and pregnenolone as well as MMP and pregnenolone after treatment with the TSPO ligands 2a and 2b as well as the molecules of reference XBD173 and SSR-180,575 in both cell lines when compared to untreated control cells (Ro5-4864 values were excluded from regression analysis). Graphs represent pregnenolone level in the abscissa versus ATP levels in the ordinate in control (Co) cells (A; confidence: 0.2754 to 1.473; R square: 0.8780; p: 0.0188) and APP cells (B; confidence: 0.1013 to 0.3502; R square: 0.9174; p: 0.0103). Graphs represent pregnenolone level in the abscissa versus MMP levels in the ordinate in control (Co) cells (C; confidence: 0.2376 to 1.075; R square: 0.8923; p: 0.0155) and APP cells (D; confidence: 0.07782 to 0.7813; R square: 0.8343; p: 0.0302). Values represent the mean of each treatment group normalized to the untreated control (=100%).
The mitochondrial oxidative phosphorylation (OXPHOS) and the cellular glycolysis are the two main pathways to produce ATP molecules. Therefore, we evaluated the efficiency of XBD173, SSR-180,575, Ro5-4864, and the TSPO ligands 2a and 2b at a concentration of 10 nM after a treatment of 24 h to modulate one or both pathways. OCR is an indicator of basal respiration, and ECAR is an indicator of glycolysis, and both were monitored simultaneously in real-time (Fig. 2). In Co cells, a significant increase of the OCR was observed after a treatment with 2b and 2a (+33% and +69% respectively) (Fig. 2A). Only the TSPO ligand 2b significantly increased the ECAR (+70%) (Fig. 2B). The bioenergetic phenotype of the Co cells (Fig. 2 C), representing OCR versus ECAR under different treatment conditions, revealed that the TSPO ligand 2b and 2a were particularly efficient to increase both parameters, switching the Co cells to a metabolically more active state.
Fig.2
Modulation of the bioenergetic phenotype by XBD173, SSR-180,575, Ro5-4864, and the new TSPO ligand 2a and 2b. A, D) Oxygen consumption rate (OCR) and (B, E) extracellular acidification rate (ECAR) were measured simultaneously using a Seahorse XF24 Analyzer in the same experimental conditions in control (Co) cells (A, B) and APP cells (D, E). Values represent the mean±SEM (n = 6 replicates) of four independent experiments. One-way ANOVA and post hoc Dunnett’s multiple comparison test versus untreated Co or APP cells, *p < 0.05, **p < 0.01, ***p < 0.001. Bioenergetic phenotype (OCR versus ECAR) of Co cells (C) and APP cells (F) revealed increased metabolic activity after treatment with the ligand TSPO 2a and 2b. Values represent the mean of each group (mean of the ECAR in abscissa/ mean of the OCR in ordinate) and were normalized to the control group (100%); OCR, Oxygen Consumption Rate (mitochondrial respiration); ECAR, Extracellular Acidification Rate (Glycolysis).
We previously showed that APP cells present a decrease of the OCR and the ECAR compared to the Co cells [36, 37]. The compounds 2b and 2a were able to increase significantly the defective OCR of APP cells (+25% and +36%, respectively) compared to the untreated group (Fig. 2D) whereas the ECAR was significantly ameliorated by XBD173 (+131%), SSR-180,575 (+136%), Ro5-4864 (+103%), and the TSPO ligand 2b and 2a (+140% and +156%. respectively) (Fig. 2E). The bioenergetic phenotype of the APP cells showed that the compounds 2b and 2a are more efficient than the molecules of references to improve the bioenergetic metabolism (Fig. 2F).
Next, we investigated more deeply the effect of XBD173, SSR-180,575, Ro5-4864, and the TSPO ligand 2b and 2a on mitochondrial respiration by measuring the OCR on permeabilized Co and APP cells after pre-treatment with the Seahorse XF PMP. PBP forms pores in cellular plasma membranes via oligomerization and it targets the cellular plasma membrane selectively, while leaving the mitochondrial membrane intact, thus allowing the control of substrate provision to the mitochondria. APP cells showed lower OCR and RCR compared to Co cells (Fig. 3A, B). We then evaluated the different respiratory states and calculated the RCR (state 3/state 4, Fig. 3 C, D) after treatment TSPO ligands. In Co cells, the RCR was significantly increased after a treatment with XBD173 (+248%), 2b (+230%), and 2a (+103%) compared to the untreated group (Fig. 3 C). In APP cells, 2a significantly increased the basal respiration (state 2, ADP-independent) with an 152% of increase and 2b with a +214% of increase compared to untreated cells (Fig. 3D). Together, in addition to ATP and pregnenolone ameliorating properties, these data demonstrate that our compounds 2b and 2a were also able to improve bioenergetic metabolism and mitochondrial respiration.
Fig.3
2b increases mitochondrial respiratory capacity in control (Co) and APP cells. Oxygen consumption rate (OCR), was measured on permeabilized control Co (C) or APP cells (D) after treatment with LTSPO for 24 h, using a XF24 Analyzer (Seahorse Bioscience). The sequential injection of mitochondrial inhibitors allows the assessment of mitochondrial respiratory state 2, state 3 (ADP-dependent) and state 4o (after oligomycin injection) (see details in the Materials and Methods section). Values corresponding to the different respiratory states (A) are represented as mean±SEM (n = 5 replicates of three independent experiments/ groups) and were normalized to the state 2 of the untreated group (=100%). C, D) The respiratory control ratio (RCR = state 3/state 4o, B), which reflects the mitochondrial respiratory capacity, was increased by XBD173, SSR-180,575, 2b, and 2a in Co cells (C) but only 2b improved the RCR in APP cells (D). One-way ANOVA and post hoc Dunnett’s multiple comparison test versus untreated Co or APP cells, *p < 0.05, **p < 0.01, ***p < 0.001.
TSPO ligands reduced Aβ levels
Our next step was to examine whether a treatment with TSPO ligands had an effect on the concentration of Aβ peptide (Aβ40) in HEK cells overexpressing the Swedish APP mutation (HEK SWE APP) cells. Aβ levels were determined in the cell supernatant using an ELISA quantification of amyloid 1–40. In HEK SWE APP cells, XBD173, SSR-180,575, 2b, and 2a were able to decrease significantly amyloid beta 1–40 levels compared to untreated HEK SWE APP cells (Fig. 4).
Fig.4
Aβ1-40 levels are significantly reduced after TSPO ligands treatment in the HEK SWE APP cells. ELISA quantification of Aβ1-40 in HEK SWE APP cells. Levels of Aβ1-40 are significantly reduced after TSPO ligands treatment. One-way ANOVA and post hoc Dunnett’s multiple comparison test versus untreated HEK SWE APP cells, *p < 0.05, **p < 0.001. ELISA, enzyme-linked immunosorbent assay; ANOVA, analysis of variance; APP, amyloid precursor protein; SWE, Swedish.
TSPO ligands decreased oxidative injury
We investigated the ability of our new TSPO ligands 2a and 2b to protect against H2O2-evoked bioenergetic abnormalities in this drastic injury condition. Energy loss, cell death, and an increase of cytosolic and mitochondrial ROS as well as the superoxide anion level in Co cells and APP cells were observed after 3 h of H2O2 treatment at 500 μM (Figs. 5 and 6). Of note and as expected, APP cells were stronger affected by the oxidative insult compared to Co cells (Figs. 5 and 6).
In Co cells, the pretreatment with the compounds 2b and 2a ameliorated ATP level (+24% and +19% respectively, Fig. 6A) compared to the cells only stressed with 500 μM of H2O2. In APP cells, similar effects were observed: 2b and 2a improved ATP levels (up to 20% of increase) compared to the cells treated only with 500 μM of H2O2 (Fig. 6D). To investigate more deeply the effect of a pre-treatment with the compounds 2a and 2b on mitochondrial respiration, RCR was calculated under oxidative conditions using permeabilized SH-SY5Y (Fig. 6B, E). In Co cells, RCR was upregulated after a pre-treatment with the compound 2b and 2a up to +89% and up to 175% of increase in APP cells compared to the cells treated with 500 μM of H2O2 alone (Fig. 6B, E). Improvement of the cell survival was observed in Co cells after a pre-treatment with the compounds 2b and 2a with respectively 2% and 4% of increase to compare to the cells treated with H2O2 (Fig. 6 C). In APP cells, only the compound 2a showed an effect on cell survival (+10% of increase) (Fig. 6F).
Finally, concerning the ROS levels, the compounds 2b and 2a reduced cytosolic ROS (–33% and –38% respectively, Fig. 5), mitochondrial ROS (–44% and –53% respectively, Fig. 5) and the superoxide anion level (–21% and –20%, respectively, Fig. 5) in Co cells when compared to cells treated with 500 μM of H2O2 alone. In APP cells, the compounds 2b and 2a reduced the cytosolic ROS (–31% and –28%, respectively, Fig. 5), mitochondrial ROS (–52% and –43%, respectively, Fig. 5), and the superoxide anion level (–33% and –35%, respectively, Fig. 5) when compared to cells treated with 500 μM of H2O2 alone.
Together, these data showed that our compounds 2b and 2a were able to protect mitochondria against oxidative injury.
Fig.5
2a and 2b pre-treatment ameliorate ATP levels, mitochondrial respiration (RCR) and cell survival after oxidative insult in control (Co) and APP cells. Co and APP cells were pre-treated with the TSPO ligand 2b or 2a for 24 h and then exposed to H2O2 (500 μM for 3 h). H2O2 treatment induces a decrease of the ATP level, RCR as well as the cell survival both cell lines. The TSPO ligand 2b or 2a improved the ATP level, RCR and cell survival. Values represent the mean±SEM; n = 4–6 replicates of three independent experiments normalized to Co or APP cells treated with H2O2. One-way ANOVA and post hoc Dunnett’s multiple comparison test versus Co or APP cells treated with H2O2, *p < 0.05, **p < 0.0001, ***p < 0.001.
Fig.6
2b and 2a decreased H2O2-induced raise of ROS in control (Co, A) and APP (B) cells. Co cells were pre-treated with 10 nM of the TSPO ligand 2b or 2a for 24 h and then exposed to H2O2 (500 μM for 3 h). H2O2 treatment induces an increase of the cytoslic ROS, mitochondrial ROS, and superoxide anion level in Co and APP cells. 2a and 2b reduced significantly the ROS generation under oxidative stress conditions. Values represent the mean±SEM; n = 4–6 replicates of three independent experiments normalized to Co or APP cells treated with H2O2. One-way ANOVA and post hoc Dunnett’s multiple comparison test versus Co or APP cells treated with H2O2. *p < 0.05, **p < 0.0001, ***p < 0.001.
DISCUSSION
In the present study, we showed that pharmacological treatment with our new TSPO ligands 2a and 2b confers protective benefit against AD-induced mitochondrial dysfunctions and oxidative injury by modulating mitochondrial bioenergetic phenotype. Ours findings demonstrate that under physiological condition, the TSPO ligands 2a and 2b were able to 1) alleviate bioenergetic deficits observed in APP/Aβ overexpressing neuroblastoma cells by activating the metabolic activity, ameliorating the mitochondrial respiration as well as raising the levels of the neurosteroid pregnenolone; 2) reduce the levels of Aβ40 in HEK SWE APP cells; and 3) under stress condition, our TSPO ligands exerted protective effects on the APP cells by ameliorating bioenergetics and reducing oxidative injury. Thus, the protective pattern of the compounds 2a and 2b are evident under physiological and oxidative stress conditions in a cellular model of AD-related amyloidopathy and with a higher effectiveness compared to TSPO ligands described in the literature (Ro5-4864, XBD173 and SSR-180,575). Indeed, our new compounds were able to increase significantly the OCR in the control and APP cells, as well as the RCR in APP cells when the other compounds showed no significant effects. This indicates a higher efficacy of our new compounds in regulating the oxidative phosphorylation-derived energy production when compared to the TSPO ligands of reference.
Based on our recent work on the discovery of imidazoquinazolinone derivatives as TSPO ligands modulating neurosteroidogenesis and ATP levels in neuroblastoma cells expressing APP [26], we continued to investigate the ability of these new TSPO ligands 2a, 2b and their molecules of references after 24 h of treatment (10 nM) to stimulate bioenergetics, and protect Co and APP cells under stress conditions. 2a and 2b represent a promising chemical family able to bind TSPO in the 2-digit nanomolar range. 2a and 2b, as well as the TSPO ligands described in the literature were able to promote ATP production, and pregnenolone biosynthesis in native SH-SY5Y cell and in cells overexpressing APP [26]. In our study, we showed that the increase in ATP levels as well as MMP levels are significantly correlated to the raise pregnenolone levels suggesting a mechanistic link between bioenergetics and pregnenolone biosynthesis. The increase in ATP levels appeared to be coupled to an increase in MMP as well as improved basal respiration. Our findings showed that only the compounds 2a and 2b significantly ameliorated the metabolic activity by improving respiration as well as glycolysis parameters, and increased the maximum capacity of respiration (RCR) of APP cells compared to the molecules of references. Furthermore, we wanted to know if the ligands 2a and 2b can protect Co and APP cells against H2O2-induced oxidative stress. We found that TSPO ligand 2a protected both cell lines by increasing ATP levels and RCR and reducing ROS. 2b improved the same parameters as the ligand 2a but is not effective enough to protect against cell death. 2a and 2b as well as XBD173 and SSR-180,575 decreased the levels of Aβ1-40. Since it is known that Aβ1-40 is able to increase oxidative stress and impair mitochondrial bioenergetics, this mechanism might contribute to the beneficial effect of TSPO ligands described here.
We propose that the neuroprotective effect of our compounds is mediated by their ability to stimulate steroid biosynthesis via TSPO agonism [38]. It is an attractive hypothesis since neurosteroid treatments with progesterone or allopregnanolone have shown beneficial effect in animal models of AD. In our recent study, 2a and 2b have been shown to stimulate the production of the precursor of the neurosteroid biosynthesis, pregnenolone [26].
Our strategy is more based on the neuroprotective effect of TSPO ligands against oxidative injury through the neurosteroidogenesis and the modulation of the bioenergetic phenotype in a cellular model of AD. In fact, the capacity to boost mitochondrial bioenergetics seems to be a common mechanism of several steroids [35]. Neurosteroids are able to improve cellular bioenergetics by ameliorating mitochondrial respiration and ATP production and regulating redox homeostasis in neuronal cells [35, 36]. In addition, a treatment with a selection of neurosteroids, namely progesterone, estradiol and testosterone reduced mitochondrial impairments induced by Aβ or abnormal protein tau [36]. TSPO ligands have been shown to promote the biosynthesis of allopregnanolone, a compound currently undergoing clinical trials as a neuroprotector to treat AD, after showing efficacy in mouse models [21]. More recently we showed that allopregnanolone and its analog BR 297 exerted neuroprotective effects to counteract AD-related bioenergetic deficits [37].
Recently, Bader and colleagues demonstrated that pregnenolone biosynthesis is dependent on TSPO expression in mouse BV-2 microglia cells [39]. In fact, they showed that XBD173 and Ro5-4864 were able to stimulate pregnenolone biosynthesis in a TSPO-dependent way and that mitochondrial function is differentially modulated by TSPO ligands [39]. The ligand-specific effects could be in a TSPO-dependent or independent manner on different cellular functions [39]. Further investigations are needed on the effect of our TSPO ligands on TSPO expression providing evidence for both specific TSPO-mediated, as well as off-target effects.
Considering the beneficial effect of TSPO ligands on neuronal viability, regeneration processes, and neuroinflammatory response, one can imagine many therapeutic uses of TSPO ligands in the central and peripheral nervous systems. XBD173 and not etifoxine elevated relevant neurosteroids in the brain at female rats and differed in its ability to exert anti-inflammatory effects. In proteolipid-protein (PLP)-induced experimental autoimmune encephalomyelitis (EAE) SJL/J-mouse model (PLP-EAE mice), XBD173 (10 mg/kg dose) increased allopregnanolone concentrations in spinal and brain tissues and decreased serum level of pro-inflammatory cytokines [40]. TSPO appears to be implicated in neuroinflammatory processes that also play a role in AD. Therefore, further investigation of pharmacological effects of TSPO ligands are warranted in AD. The effects of benzimidazole derivatives (modulators of mitochondrial activity) were recently tested for their ability to restore cells from Aβ-induced toxicity in vitro in HT22 cells (mouse hippocampal cells) and in vivo as a potential treatment for AD [41]. Among these compounds, one benzimidazole derivative was able to alleviate Aβ-induced mitochondrial dysfunction in cells by recovering the mitochondrial membrane potential, ATP production, cellular viability, and suppressing ROS in vitro as well as to improve cognitive function in animal models of AD. In this study, Kim and collaborators developed novel benzimidazole derivatives as an mPTP blocker to treat mitochondrial dysfunction in AD [41].
Of note, in the present work, we used neuroblastoma SH-SY5Y cells stably transfected with the human wild-type APP, a cellular model well established which possesses various characteristics found in AD pathology, including increased Aβ production, ROS generation, and impaired mitochondrial function (decrease of ATP production, mitochondrial respiration, and mitochondrial complex IV activity) [36, 42, 43]. Interestingly, it has also been demonstrated that APP/Aβ-overexpression causes abnormal mitochondrial morphology and distribution in neuroblastoma M17 cells, suggesting the possible occurrence of morphological alterations of mitochondria in APP/Aβ SH-SY5Y cells [44]. Nevertheless, since SH-SY5Y cells are not as highly dependent on the oxidative phosphorylation (OXPHOS) as primary cell cultures to produce ATP, we further need to investigate the mechanism of action of our TSPO ligands in other models, such as primary cell cultures [45].
Taking together, our results convincingly demonstrate that the new imidazoquinazolinone TSPO ligands protect against oxidative stress, induce the de novo synthesis of neurosteroids, improve cellular bioenergetics, and reduce ROS and Aβ levels, suggesting that these compounds could be potential new therapeutic tools for the treatment of AD.
ACKNOWLEDGMENTS
Parts of this were performed in the frame of a joint PhD thesis work (IL) co-supervised by AGMN and AE between the University of Strasbourg (France) and the University of Basel (Switzerland) that was part of the collaborative research program of the NeuroRhine Consortium that was funded by INTERREG IV Program (European Fund for Regional Development) in the Upper Rhine Region and the Offensive Science Call 2012. Additional Research Funds were from the Psychiatric University Clinics (UPK research Fonds) and the Swiss National Science Foundation (SNF#31003A_149728, to AE).
Authors’ disclosures available online (https://www.j-alz.com/manuscript-disclosures/19-0127r1).
SUPPLEMENTARY MATERIAL
[1] The supplementary material is available in the electronic version of this article: https://dx.doi.org/10.3233/JAD-190127.
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