Affiliations: [a] Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX, USA
| [b] Centre for Neuroscience, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, City of Knowledge, Republic of Panama | [c] Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
| [d] College of Sciences, The University of Texas at San Antonio, San Antonio, TX, USA
| [e] Houston Methodist Neurological Institute, Institute of Academic Medicine, Houston Methodist Hospital, Houston, TX, USA
| [f]
Weill Cornell Medical College of Cornell University, New York, NY, USA
Correspondence:
[*]
Correspondence to: Muralidhar L. Hegde, Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030, USA. E-mail: mlhegde@houstonmethodist.org. and K.S. Rao, Centre for Neuroscience, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, City of Knowledge, Republic of Panama. E-mail: jrao@indicasat.org.pa.
Note: [1] The pCW-FLAG-(inducible)-α-Syn plasmid is being made available in Addgene and the pCW-iFLAG-α-Syn SH-SY5Y cell line is available upon direct request to MLH.
Abstract: Altered expression of α-synuclein is linked to Parkinson’s disease (PD). A major challenge to explore how the increased α-synuclein affect neurotoxicity is the lack of a suitable human neuronal cell model that mimics this scenario. Its expression in neural precursors affects their differentiation process, in addition to the neuronal adaptability and variability in maintaining a constant level of expression across passages. Here, we describe an SH-SY5Y line harboring Tet-ON SNCA cDNA cassette that allows for induction of controlled α-synuclein expression after neuronal differentiation, which can be an important tool for PD research.
Keywords: α-synuclein, dopaminergic neurons, inducible α-synuclein expressing neuronal model for PD, Parkinson’s disease
