Association Studies of Specific Cholesterol Related Genes (APOE, LPL, and CETP) with Lipid Profile and Memory Function: A Correlative Study Among Rural and Tribal Population of Dharmapuri District, India

Article type: Research Article

Authors: Periyasamy, Sabapathy^{a; 1} | Sathya, Mohan^a | Karthick, Chennakesavan^a | Kandasamy, Mahesh^b | Shanmugaapriya, Sellathamby^c | Tamilselvan, Jeyavelu^d | Jayachandran, Kesavan Swaminathan^e | Anusuyadevi, Muthuswamy^{a; *; 1}

Affiliations: [a] Department of Biochemistry, Molecular Gerontology Laboratory, Bharathidasan University, Tiruchirappalli, India | [b] UGC-Faculty Recharge Program (UGC-FRP), University Grant Commission, Laboratory of Stem cells and Neuroregeneration, Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India | [c] Department of Bio-Medical Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India | [d] Center for Biotechnology, Anna University, Chennai, Tamilnadu, India | [e] Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, Tamilnadu, India

Correspondence: [*] Correspondence to: Muthuswamy Anusuyadevi, Assistant Professor, Department of Biochemistry (DST-FIST Sponsored), Molecular Gerontology Laboratory, Bharathidasan University, Tiruchirappalli, Tamilnadu 620024, India. Tel.: +91 431 2407071/Extn: 438; Fax: +91 431 2407045; E-mail: msanushyas2005@gmail.com.

Note: [1] These authors contributed equally to this work

Abstract:  Epidemiological studies state that dementia has multiple etiologies including genetic mutation, genetic variation, and environmental factors. Accumulating evidence suggests that dysregulation of cholesterol homeostasis is the major etiological factor in initiating neurodegeneration. Apolipoprotein E (APOE) polymorphic alleles and associated polymorphism of lipoprotein lipase (LPL) and cholesteryl ester transfer protein (CETP) that are important components in regulating cholesterol metabolism are implicated in neurodegenerative diseases. Therefore, the current study focused on identifying the association between several common polymorphism (viz., APOE, CETP, and LPL) to that of change in serum lipid levels and memory symptoms. Volunteer subjects aged 50 and above from rural and tribal areas of the Dharmapuri district, Tamilnadu, India were chosen for the current study and polymorphism was analyzed using PCR-RFLP. Fasting lipid profile and memory function using simplified version of Global Clinical Dementia rating were assessed. Significant difference in the major lipid profile parameters were observed (TC, TGL, LDL, VLDL) among rural and tribal populations that were associated with significant genotypic variation of APOE, CETP, and LPL. Regression analysis revealed significant risk for memory loss that are dependent on age and genetic variants like CETP. These data predict positive correlation between cholesterol-associated genes and their relationship to altered lipid profile and memory symptoms, which possibly link gene-polymorphism and susceptibility ratio for aging and dementia.

Keywords: Alzheimer’s disease, apolipoprotein E, cholesteryl ester transfer protein, lipid profile, lipoprotein lipase, memory, polymorphism

DOI: 10.3233/JAD-170272

Journal: Journal of Alzheimer's Disease, vol. 60, no. s1, pp. S195-S207, 2017