BDNF, TNFα, HSP90, CFH, and IL-10 Serum Levels in Patients with Early or Late Onset Alzheimer's Disease or Mild Cognitive Impairment

Article type: Research Article

Authors: Gezen-Ak, Duygu^a | Dursun, Erdinç^a | Hanağası, Haşmet^b | Bilgiç, Başar^b | Lohman, Ebba^b | Araz, Ömür Selin^a | Atasoy, İrem L.^a | Alaylıoğlu, Merve^a | Önal, Burak^a | Gürvit, Hakan^b | Yılmazer, Selma^{a; *}

Affiliations: [a] Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey | [b] Behavioral Neurology and Movement Disorders Unit, Department of Neurology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

Correspondence: [*] Correspondence to: Prof. Dr. Selma Yilmazer, Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey. Tel.: +90 212 414 30 00/21627; Fax: +90 212 414 30 42; E-mail: drselmayilmazer@gmail.com.

Abstract: Identifying early-detection biomarkers have become an increasingly important approach in the treatment and prevention of Alzheimer's disease (AD). In this study, we investigated the potential of brain-derived neurotrophic factor (BDNF), complement factor H (CFH), tumor necrosis factor-α (TNFα), interleukin 10 (IL-10), and heat shock protein 90 (Hsp90) as serum biomarkers for AD in a cohort of the Turkish population because they have been suggested to be associated with AD. Serum BDNF, CFH, TNFα, IL-10, and Hsp90 levels in three groups of patients, early-onset AD (EOAD; age of onset < 65; n = 22), late-onset AD (LOAD; age of onset > 65; n = 54), and mild cognitive impairment (MCI) (n = 30), were compared with age-matched healthy controls (age < 65, n = 18 and age > 65; n = 32) using ELISA. The serum BDNF levels significantly decreased and TNFα levels significantly increased in the EOAD and LOAD groups compared to the age-matched healthy controls. There was a correlation between serum TNFα and IL-10 levels in the LOAD and healthy control groups. Serum CFH levels in the LOAD and MCI patients were significantly decreased compared with controls. Serum Hsp90 levels in the EOAD, LOAD, and MCI patients were significantly decreased compared with controls. The protein misfolding, the inflammatory response, and decreased neurotrophic factor synthesis are all suggested to be related to AD type brain pathology, and our results indicate these alterations might be traced from serum samples. For accurate early diagnosis of AD, it is important to determine a profile of alterations in multiple biomarkers in large-scale population studies.

Keywords: Alzheimer's disease, brain-derived neurotrophic factor (BDNF), complement factor H (CFH), ELISA, heat shock protein 90 (Hsp90), interleukin 10 (IL-10), mild cognitive impairment (MCI), tumor necrosis factor-α (TNFα)

DOI: 10.3233/JAD-130497

Journal: Journal of Alzheimer's Disease, vol. 37, no. 1, pp. 185-195, 2013