Affiliations: [a] Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA | [b] Department of Psychiatry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA | [c] Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA | [d] Institute for Basic Research in Developmental Disabilities, Department of Developmental Neurobiology, Division of Immunology, Staten Island, NY, USA
Correspondence:
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Correspondence to: Esther S. Oh: Department of Medicine, Division of Geriatric Medicine and Gerontology, 5505 Hopkins Bayview Circle, Baltimore, Maryland 21224, USA. Tel.: +1 410 550 1318; Fax: +1 410 550 8701; E-mail: eoh9@jhmi.edu.
Note: [] Handling Associate Editor: Ralph Martins
Abstract: Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.
