Affiliations: [a] The Birchall Centre, Lennard-Jones Laboratories, Keele University, Staffordshire, UK | [b] School of Engineering, Warwick University, Coventry, UK | [c] Life Sciences, Keele University, Staffordshire, UK
Correspondence:
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Corresponding author: Dr. Christopher Exley, The Birchall Centre, Lennard-Jones Laboratories, Keele University, Staffordshire, ST5 5BG, UK. E-mail: c.exley@chem.keele.ac.uk
Abstract: The observation of the co-deposition of metals and amyloid-β42 (Aβ42) in brain tissue in Alzheimer's disease prompted myriad investigations into the role played by metals in the precipitation of this peptide. Copper is bound by monomeric Aβ42 and upon precipitation of the copper-peptide complex thereby prevents Aβ42 from adopting a β-sheet secondary structure. Copper is also bound by β-sheet conformers of Aβ42, and herein we have investigated how this interaction affects the conformation of the precipitated peptide. Copper significantly reduced the thioflavin T fluorescence of aged, fibrillar Aβ42 with, for example, a 20-fold excess of the metal resulting in a ca 90% reduction in thioflavin T fluorescence. Transmission electron microscopy showed that copper significantly reduced the quantities of amyloid fibrils while Congo red staining and polarized light demonstrated a copper-induced abolition of apple-green birefringence. Microscopy under cross-polarized light also revealed the first observation of spherulites of Aβ42. The size and appearance of these amyloid structures were found to be very similar to spherulites identified in Alzheimer's disease tissue. The combined results of these complementary methods strongly suggested that copper abolished the β-sheet secondary structure of pre-formed, aged amyloid fibrils of Aβ42. Copper may protect against the presence of β-sheets of Aβ42 in vivo, and its binding by fibrillar Aβ42 could have implications for Alzheimer's disease therapy.
Keywords: Aluminum, Alzheimer's disease, amyloid, Aβ42, β-sheet, Congo red, copper, spherulites, TEM, thioflavin T
