Alzheimer's Amyloid-β Rescues Yeast from Hydroxide Toxicity

Article type: Short Communication

Authors: Dubey, Ashok K.^{a; c} | Bharadwaj, Prashant R.^{a; b; *} | Varghese, Joseph N.^a | Macreadie, Ian G.^{a; b}

Affiliations: [a] CSIRO Molecular and Health Technologies and Preventive Health Flagship, Parkville, VIC, Australia | [b] Centre of Excellence for Alzheimer's Disease Research and Care, School of Exercise, Biomedical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia | [c] Netaji Subhash Institute of Technology, New Delhi, India

Correspondence: [*] Corresponding author. Prashant R. Bharadwaj, E-mail: prashant.bharadwaj@csiro.au.

Abstract: Amyloid-β (Aβ42), which is known to be toxic to neuronal cells, protects yeast cells from severe sodium hydroxide toxicity. More than 85% cell death was caused by treatment with 1 mM NaOH and approximately 95% was observed at a 2 mM concentration. However, greater than 55% cells survived the treatment in the presence of Aβ42. A strong protective effect of the peptide was also evident from the differential staining of the treated culture with propidium iodide.

Keywords: Alkali stress, amyloid-β protection, amyloid-β toxicity, peptide assay, yeast assay

DOI: 10.3233/JAD-2009-1121

Journal: Journal of Alzheimer's Disease, vol. 18, no. 1, pp. 31-33, 2009