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Article type: Research Article
Authors: Nagababu, Enikaa; | Usatyuk, Peter V.b; | Enika, Divyab | Natarajan, Viswanathanb | Rifkind, Joseph M.a; *
Affiliations: [a] Molecular Dynamics Section, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA | [b] Pulmonary and Critical Care Section, Department of Medicine, The University of Chicago, Chicago, IL, USA
Correspondence: [*] Corresponding author: Dr. Joseph M. Rifkind, Molecular Dynamics Section, National Institute on Aging, National Institutes of Health, 251 Bayview Blvd, Baltimore, MD 21224, USA. Tel.: +1 410 558 8168; Fax: +1 410 558 8397; E-mail: rifkindj@mail.nih.gov.
Note: [1] These authors contributed equally to this work.
Note: [] Communicated by Jack de la Torre
Abstract: Neuronal inflammation is very common in Alzheimer's disease (AD). This inflammation can be caused by infiltration of neutrophils across the blood brain barrier. Endothelial permeability changes are required for the infiltration of high molecular weight components to the brain. Deposition of toxic amyloid-β (Aβ) fibrils in the cerebral vasculature, as well as in brain neurons, has been implicated in the development of AD. This study investigates the effect of Aβ fibrils on the permeability of the endothelium and the mechanism for the observed permeability changes. Aβ1-40 and Aβ1-42 fibrils, but not monomers, were found to increase permeability of bovine pulmonary arterial endothelial cells in a dose- and time dependent manner as detected by transendothelial electrical resistance. This increase in permeability is only partially (25%) inhibited by catalase and is not associated with an increase in cytosolic Ca+2 or tyrosine phosphorylation. These results indicate that hydrogen peroxide is not the primary mediator for the permeability changes. Treatment of cells with both amyloid fibrils resulted in stress fiber formation, disruption and aggregation of actin filaments, and cellular gap formation. The results of this study reveal that Aβ increases the permeability of endothelium by inducing change in the cytoskeleton network.
Keywords: Alzheimer's disease, amyloid-β, endothelial cells, permeability
DOI: 10.3233/JAD-2009-1104
Journal: Journal of Alzheimer's Disease, vol. 17, no. 4, pp. 845-854, 2009
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