Potentiometric biosensors based on ISFETs and immobilised cholinesterases

Issue title: Second INABIO Symposium, 2005

Article type: Research Article

Authors: Dzyadevych, Sergei V.^{a; b; *} | Arkhypova, Valentyna N.^{a; b} | Martelet, Claude^b | Jaffrezic-Renault, Nicole^b | Chovelon, Jean-Marc^c | El'skaya and, Anna V.^a | Soldatkin, Alexey P.^a

Affiliations: [a] Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, 150 Zabolotnogo St., Kiev 03143, Ukraine | [b] Ecole Centrale de Lyon, CEGELY, UMR CNRS 5005, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France | [c] Laboratoire d'Application de la Chimie à l'Environnement, CNRS-Université Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France

Correspondence: [*] Corresponding author. Tel.: +380 44 266 07 49; Fax: +380 22 266 07 59; E-mail: dzyad@yahoo.com

Abstract: This paper describes authors' achievements in the development, investigation, and application of cholinesterase biosensors based on ISFETs. Various biosensors for determination of concentrations of different toxic substances (organophosphorous and carbamate pesticides, hypochlorite, glycoalkaloids) were designed on the basis of reversible and/or irreversible enzyme inhibition effects. The main analytical characteristics of the biosensors developed have been studied under different conditions and optimal experimental protocols for toxic substances determination have been proposed. Most of these biosensors show a high reproducibility and a good operational and storage stability. A quite good correlation with results obtained through routinely used standard methods as HPLC has been shown.

Keywords: ISFETs, cholinesterases, inhibition, pesticides, glycoalkaloids

DOI: 10.3233/JAE-2006-740

Journal: International Journal of Applied Electromagnetics and Mechanics, vol. 23, no. 3-4, pp. 229-244, 2006