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Article type: Research Article
Authors: Vollmers, H. Petera; * | Wozniak, Ewaa | Stepien-Bötsch, Ewaa | Zimmermann, Ulrichb | Müller-Hermelink, Hans-Konrada
Affiliations: [a] Institut für Pathologie, Universität Würzburg, Josef-Schneider Würzburg, Germany | [b] Institut für Biotechnologie, Universität Würzburg, Am Hubland, Würzburg, Germany
Correspondence: [*] Correspondence and reprint requests to: Dr H. Peter Vollmers, Institut für Pathologie, Universität Würzburg, Josef-Schneider Str. 2, 97080 Würzburg, Germany.
Abstract: We describe in this paper the rapid and cheap purification of human immunoglobulin M from hybridoma supernatant of mass culture. The method consists of two steps: 1. concentration of supernatant by ultrafiltration and 2. dialysis against distilled water, pH 6.4. To produce the supernatant, the hybridomas are grown in RPM1 media with fetal calf serum (10% FCS) in 250 ml flasks under normal tissue culture conditions. More than 14 mg IgM can be nearly selectively purified within 6 h from 5 l of antibody containing hybridoma supernatant. Most of the IgM molecules stay in a penta- and monomeric form and are positive in physiological and immunohistochemical studies.
Keywords: Human immunoglobulin M, immunoglobulin mass culture, water dialysis, pure human IgM
DOI: 10.3233/HAB-1996-7105
Journal: Human Antibodies, vol. 7, no. 1, pp. 37-41, 1996
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