Comparison of three human-murine heteromyeloma cell lines for formation of human hybridomas after electrofusion with human peripheral blood lymphocytes from meningococcal cases and carriers

Article type: Research Article

Authors: Delvig, Alexei A.^{a; ;} | Koumare, Brehima^b | Glasert, Ralf W.^c | Wang, Jian-Fu^a | Jahn, Sigbert^b; | Achtman, Mark^a

Affiliations: [a] Max Planck Institut für Molekulare Genetik, Berlin, Germany | [b] Institut National de Recherche en Santé Publique, Bamako, Mali | [c] Institute Jor Medical Immunology Charité, Humboldt University, Germany

Correspondence: [] Correspondence and reprint requests to: Dr Alexei Delvig, Department of Vaccines, National Institute of Public Health (Folkehelsa), Geitmyrsveien 75, N-0462 Oslo, Norway.

Note: [] Present addresses: (A.D.) National Institute of Public Health, Department of Vaccines, Geitmyrsveien 75, N-0462 Oslo, Norway. (S.J.) Hautklinik Charite, Humboldt University, D-I0117 Berlin, Germany.

Abstract: Peripheral blood lymphocytes from meningitis patients and healthy meningococcal carriers were fused by electro fusion with the three human-murine heteromyeloma cell lines CB-F7, K6H6B5 and H7NS. 934 hybridomas producing human immunoglobulins were obtained in 30 fusions. Heteromyeloma K6H6B5 yielded a significantly higher proportion of hybridomas producing IgG antibodies than did the two other cell lines. CB-F7 and K6H6B5 yielded comparable numbers of hybridomas whose supernatants reacted with homologous bacteria, whereas the cell line H7NS was less efficient.

Keywords: Electrofusion, human monoclonal antibodies, heteromyeloma cells, meningococci

DOI: 10.3233/HAB-1995-6201

Journal: Human Antibodies, vol. 6, no. 2, pp. 42-46, 1995