Affiliations: [a] Department of Medical Laboratory Science, Komar University of Science and Technology, Sulaymaniyah, Kurdistan region, Iraq | [b] Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, Pécs University, Pécs, Hungary | [c] Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Correspondence:
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Corresponding authors: Leili Aghebati-Maleki, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Tel.: +98 413 3364665; Fax: +98 41 3364665; E-mail: aghebatil@ tbzmed.ac.ir. Hamid Ahmadi, Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, Pécs University, Pécs, Hungary. E-mail: ahmadi.hamid@aok.pte.hu.
Abstract: BACKGROUND: Immunoassay methods typically involve the use of antibodies, which are either labeled with an enzyme to generate a detectable product or directly tagged with a radioactive or fluorescent substrate. METHODS: One approach to enhance the specificity of immuno-detection methods is by employing a combination of different antibodies, such as primary and secondary. RESULTS: However, relying solely on one antibody targeting another may not offer the highest level of precision for improving immunoassay specificity; A novel strategy for enhancing the specificity of immunoassay techniques involves directly targeting different epitopes of an antigen. CONCLUSIONS: This approach entails utilizing sequential chain reactions facilitated by distinct enzymes bound to various antibodies, each directed at specific epitopes on the antigen. Such an innovative method holds promise for advancing the specificity of immunoassay methods.
