Affiliations: [a] Department of Education, Medical Laboratory Science Council of Nigeria, Abuja, Nigeria | [b] Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria | [c] Medical Microbiology Division, Medical Laboratory Services, University of Benin Teaching Hospital, Benin City, Nigeria | [d] Department of Physiology, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria | [e] School of Medical Laboratory Sciences, University of Benin Teaching Hospital, Benin City, Nigeria
Correspondence:
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Corresponding author: Richard Omoregie, Medical Microbiology Division, Medical Laboratory Services, University of Benin Teaching Hospital, Benin City, Nigeria. Tel.: +234 8105543053; E-mail: richard.omoregie@ubth.org.
Abstract: BACKGROUND: Morbidity and mortality associated with HIV infection is immune-mediated, and an understanding of HIV immunology will be beneficial in the management of HIV infection OBJECTIVE: The objective of this research was to measure the levels of TNF-α, IL-6 and IFN-γ in asymptomatic HIV patients and non-HIV subjects, as well as their relationship with CD4 count. METHOD: Blood samples were collected from 173 subjects, consisting of 125 asymptomatic HIV patients (44 HAART-naïve and 81 on HAART) and 48 non-HIV subjects. The IFN-, IL-6, and TNF- levels in the blood were determined using enzyme-linked immunosorbent assays, and the CD4 count of all participants was determined using flow cytometry. RESULTS: Regardless of treatment status, the IFN-γ levels of non-HIV subjects were significantly higher than those of HIV patients (p< 0.001). The opposite was true for IL-6, as the levels of IL-6 in non-HIV subjects were significantly lower than those in HAART-naïve HIV patients (p< 0.001) and those on HAART (p< 0.01). TNF-α levels did not differ between HIV patients and their non-HIV counterparts. Generally, the levels of these cytokines was not affected (p> 0.05) by immunosuppression (measured by CD4 count < 200 cells/μL) and there was no significant correlation between CD4 count and these cytokines (p> 0.05). CONCLUSION: In conclusion, asymptomatic HIV infection decreased IFN-γ, increased IL-6, and had no effect on TNF-α levels, regardless of treatment status. Immunosuppression had no impact on these cytokine levels, and there was no relationship between them and CD4 counts.
Keywords: Cytokines, asymptomatic, HIV patients, CD4 count
