The effect of T helper (Th)/T cytotoxic (Tc) ratio on disease severity in type A hemophilia patients

Article type: Research Article

Authors: Khazaei, Hossein Ali^a | Naderi, Majid^{b; *} | Aliabad, Ghasem Miri^c | Tabatabaei, Seyed Mohammad Nasiraldin^a | Alidadi, Ali^a | Moulaei, Nezar Ali^d | Safdari, Mohammad^a | Nahvi, Hedayatollah^e | Cohan, Nader^f | Dehghan, Javid^g | Mehraban, Ahmad^h | Jalili, Armanⁱ | Khazaei, Amin^a | Khazaei, Ehsan^a | Khazaei, Bahman^a | Khazaei, Behnam^a | Rezaei, Nima^j | Salarzaie, Morteza^k | Soleimani, Gholamreza^c

Affiliations: [a] Clinical Immunology Research Center (CIRC), Zahedan University of Medical Sciences, Zahedan, Iran | [b] Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | [c] Children and Adolescents Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | [d] Infectious Disease and Tropical Medicine Research Center, Resistance Tuberculosis Institute of Zahedan University of Medical Sciences, Zahedan, Iran | [e] Department of Pediatric Surgery, Tehran University of Medical Sciences, Tehran, Iran | [f] Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | [g] Community Medicine Department, Zahedan University of Medical Sciences, Zahedan, Iran | [h] Islamic Azad University, Zahedan Branch, Zahedan, Iran | [i] Department of Sport Physiology, Zahedan University of Farhangian, Zahedan, Iran | [j] Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran | [k] Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran

Correspondence: [*] Corresponding author: Majid Naderi, Genetics of Non-Commun- icable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. E-mail: thn.search@gmail.com.

Abstract: BACKGROUND AND OBJECTIVES: The aim of this study was to investigate the T helper (Th) to T cytotoxic (Tc) ratio in children suffering from type A hemophilia disease and to evaluate the correlation of this ratio with disease severity. MATERIAL AND METHOD: Two mls of EDTA anti coagulated whole blood was collected. Immunophenotyping of lymphocytes count was carried out by FACS analysis using a double CD4 and CD8 kit. The mean ± SD of absolute numbers of CD4 and CD8 lymphocytes/ml was calculated and the ratio of CD4/CD8 was evaluated by statistical method. RESULTS: Among 80 type A hemophilia patients, 66 (82.5%) were male. The mean age was 15 ± 3.51 years. 12 (15%) of them were suffering from mild disease and 68 (85%) had sever disease. The CD4 /CD8 ratio was obtained between 0.45 and 1.44 with mean1.79 ± 0.78. The correlation between this ration and disease severity was 0.019. CONCLUSION: The results showed that CD4/CD8 ratio has correlation with disease severity in type A hemophilia patients, however there was no association between this ratio and gender.

Keywords: CD4/CD8 ratio, hemophilia disease

DOI: 10.3233/HAB-200434

Journal: Human Antibodies, vol. 29, no. 1, pp. 95-99, 2021