Identification of HLA-A/B/DRB1 alleles in Iranian patients with Fanconi anemia

Article type: Research Article

Authors: Sayad, Arezou^a | Dehaghi, Mohammadreza Ostadali^{b; c} | Taheri, Mohammad^d | Fallah, Hamid^{a; e} | Arsang-Jang, Shahram^f | Shadnoush, Mahdi^g | Ghafouri-Fard, Soudeh^{a; *} | Hamidieh, Amir Ali^{h; *}

Affiliations: [a] Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [b] Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran | [c] Hematology, Oncology, and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran | [d] Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [e] Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [f] Clinical Research Development Center, Qom University of Medical Sciences, Qom, Iran | [g] Department of Clinical Nutrition, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [h] Pediatric Cell Therapy Research Center, Tehran University of Medical Sciences, Tehran, Iran

Correspondence: [*] Corresponding authors: Soudeh Ghafouri-Fard, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Tel./Fax: +98 2123872572; E-mail: s.ghafourifard@ sbmu.ac.ir; Amir Ali Hamidieh, Pediatric Cell Therapy Research Center, Tehran University of Medical Sciences, Tehran, Iran. E-mail: aahamidieh@sina.tums.ac.ir.

Abstract: Fanconi anemia includes a number of clinically and genetically diverse disorders all of them being associated with genomic instability. Some previous studies reported higher frequencies of certain HLA alleles in patients with Fanconi anemia. In the current study, we genotyped HLA-A/B/DRB1 alleles in 40 Iranian patients with Fanconi anemia. We also genotyped these alleles in the same number of Iranian sex-matched healthy individuals. The frequency of DRB1*11 was significantly higher in patients compared with controls (OR (95% CI) = 2.143 [1.05, 4.46], P value = 0.036). On the other hand, the frequencies of DRB1*13 and B*13 were lower in patients compared with controls (OR (95% CI) = 0.134 [0.02, 0.55], P value = 0.003 and OR (95% CI) = 0.13 [0.01, 0.89], P value = 0.035, respectively). Assessment of genetic divergence using Fstat test showed complete divergence in HLA-A, -B, -DRB1 alleles and haplotypes between patients and controls. The current study provides evidences for different distribution of HLA alleles between patients with Fanconi anemia and healthy subjects.

Keywords: Fanconi anemia, HLA-A/B/DRB1 alleles, HLA haplotypes

DOI: 10.3233/HAB-200410

Journal: Human Antibodies, vol. 28, no. 3, pp. 221-226, 2020