Affiliations: [a] Molecular Medicine Group, Medical Biotechnology Deapartement, National Institute of Genetics and Biotechnology, Tehran, Iran | [b] Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran | [c] Faculty of Biological Sciences, Tarbiat Modarres University, Tehran, Iran
Correspondence:
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Corresponding author: Jamshid Raheb, Institute of Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. Tel.: +98 919 796 2203; E-mail: jam@nigeb.ac.ir.
Abstract: Studies on the blood of patients with prostate cancer using Dynamic Light Scattering (DLS) and corona protein size changes have shown that this test is highly specific and sensitive, but this method has not been studied in Iran, and therefore this study intends to perform this procedure using gold nanoparticles in prostate cancer detection. Blood samples of 60 male subjects aged 40–90 years were collected from 20 healthy, 20 benign and 20 prostate cancer patients. Optical scattering changes were measured by the level of gold nanoparticles mixed with these sera, and the responses were compared with the PSA index (Prostate Specific Antigen) of the subjects. Results of D2/D1 ratio analysis were performed using SPSS statistical software R. No significant differences were found in the size of the corona protein structure between the three groups of males with cancer, males with benign tumor, and healthy males. No correlation was found between the light scattering concentration and PSA serum level Due to changes in ambient temperature, prolonged test duration or high IgG levels in apparently healthy individuals, this test is not feasible in Iran. Performing this test requires advanced equipment to maintain the same temperature that do not exist in Iran. DLS also has major limitations for prostate cancer detection, so it cannot be a simple and accurate method for the early detection of prostate cancer, and it is suggested that other methods be used to diagnose.
Keywords: Gold nanoparticles, cancer, prostate, early detection, active light scattering
