Surface modification of superparamagnetic iron oxide (SPION) and comparison of cytotoxicity effect of mPEG2000-PEI-SPION and mPEG750-PEI-SPION on the human embryonic carcinoma stem cell, NTERA2 cell line
Affiliations: [a] National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | [b] Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence:
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Corresponding authors: Mehdi Mohammadi, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. %****␣hab-28-hab200403_temp.tex␣Line␣25␣**** E-mail: m.mohammadi@nigeb.ac.ir; Jamshid Raheb, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. E-mail: Jam@nigeb.ac.ir.
Abstract: Non-viral carriers based on nanoparticles are promising vectors for drug and gene delivery into the target cells. The data provided in this article are related to research entitled “Efficiency of surface modified SPION”. In this article the surface of superparamagnetic iron oxide (SPION) (core) coated with poly (ethylene glycol)-grafted polyethylenimine (mPEG-co-PEI) shell. PEI was used to increase gene transfection efficiency and poly (ethylene glycol) methyl ether was applied to reduce cytotoxicity of nanoparticles, because our goal is that two sets of mPEG-co-PEI coated SPIONs (mPEG-750 and 2000) were prepared as carrier for the purpose of gene delivery. Structure of the mPEG-co-PEI product was elucidate by using 1H-NMR spectroscopy. Physicochemical features of the modified-SPIONs were evaluated by zeta-potential analysis. Cytotoxic effect of Nano carries were then assayed by MTT in NT2 cell line. Data analyzed by excel and p< 0.05 was considered significant. Finally siRNA absorption Ability of mPEG750-PEI-SPION and mPEG2000-PEI-SPION was tested by N/P ratio test (gel retardation assay). Our data shown that mPEG750-G-Pei-Spion and mPEG2000-G-Pei-Spion were non-toxic up to 100 μg/ml in vitro for NT2 cell line.
