Affiliations: [a] Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [b] Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | [c] Iran University of Medical Sciences, Tehran, Iran | [d] Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence:
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Corresponding authors: Arezou Sayad, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Tel./Fax: +98 2123872572; E-mail: ar.sayad@sbmu.ac.ir; Mohammad Taheri, Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Tel./Fax: +98 2123872572; E-mail: mohammad_823@yahoo.com.
Abstract: Celiac disease (CD) is a common autoimmune disease that is manifested by inflammation of the small intestine and varying extra intestinal symptoms, also considered to be associated with human HLA-DQ genes. In this study, 40 patients of CD and 40 healthy control samples were genotyped for HLA-DQB1 and 14 patients of CD and 14 healthy control samples were genotyped for HLA-DQA1genes using the SSP-PCR technique and a commercial kit. The DQA1*05 allele had the highest frequency among the patient group (42.86%). The frequency of this allele was 28.57% in healthy controls, and there was no statistically significant difference in this case (p= 0.771). The DQB1*02 allele was the most common in patients (33.75%) followed by the DQB1*03 allele (31.25%). The difference in frequency of the HLA-DQB1*02 allele in the patient and control groups was statistically significant (P= 0.0002, OR = 4.72). The remarkable differences in the distribution of HLA-DQ2 in Iranian patients compared to controls and relative risks signified the role of these alleles in the development of CD in Iranian patients and confirmed the likelihood of using HLA-DQ typing in the substantiation of the disease.
