Epitope analysis of Japanese cedar pollen allergen Cry j1 with the human monoclonal antibody 4701-1

Article type: Research Article

Authors: Naganawa, Yasunori^a | Takeda, Mio^a | Shimmoto, Michie^b | Shinmoto, Hiroshi^{a; *}

Affiliations: [a] College of Agriculture, Tamagawa University, Machida, Tokyo, Japan | [b] Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan

Correspondence: [*] Corresponding author: Hiroshi Shinmoto, Tamagawa University, College of Agriculture, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan. Tel./Fax: +81 42 739 8267; E-mail: shnmt920@agr.tamagawa.ac.jp

Abstract: We obtained a stable human-mouse hybridoma clone 4701-1 secreting IgM class human monoclonal antibody to Japanese cedar pollen allergen Cry j1. A pin-peptide enzyme-linked immunosorbent assay (ELISA) with synthesized pentadeca peptides showed a peptide with an amino acid sequence of LYTVT NSDDD PVNPA was found to be positive. Detailed analysis with deca to tetra peptides indicated that an amino acid sequence of TVTN was an essential sequence for antibody binding. When N (Asn) was substituted with A (Ala) of the TVTN epitope, the resulting peptide did not have antibody binding ability. We concluded that the TVTN sequence might have a very important role in early recognition of Cry j1 allergen by Cry j1-specific B cells, which act as antigen presenting cells.

Keywords: Human monoclonal antibody, allergen, Japanese cedar pollen, epitope, IgM

DOI: 10.3233/HAB-140273

Journal: Human Antibodies, vol. 22, no. 3-4, pp. 73-76, 2013