Selection of naturally occurring autoantibodies to interleukin-18 from phage display library

Issue title: Abstracts of HAH 2010, April 14–16, 2010, Porto, Portugal

Article type: Research Article

Authors: Vikhrova, M.A.^a | Shveygert, M.V.^b | Khrapov, E.A.^a | Filipenko, M.L.^a | Gileva, I.P.^c | Morozova, V.V.^a | Tikunova, N.V.^{a; *}

Affiliations: [a] Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | [b] Novosibirsk State University, Novosibirsk, Russia | [c] State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk region, Russia

Correspondence: [*] Corresponding author: N.V. Tikunova, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk, 630090, Russia. Tel.: +7 383 363 5157; Fax: +7 383 363 515; E-mail: tikunova@niboch.nsc.ru.

Abstract: Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.

Keywords: Naïve phage display library, human scFv, human IL-18, naïve autoantibodies

DOI: 10.3233/HAB-2010-0230

Journal: Human Antibodies, vol. 19, no. 2-3, pp. 71-78, 2010