Improved fusion partners transfected with DNA fragment encoding IL-11 on generation of human B lymphocyte hybridomas

Article type: Research Article

Authors: Zhu, Yong | Jin, Boquan | Jiang, Shaozhun | Sun, Kai | Sun, Chen | Liu, Xuesong

Affiliations: Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi-710032, P.R. China. E-mail: zhuyong@ihw.com.cn

Abstract: IL-11, a less identified cytokine, possesses some overlapping functions with IL-6 that are able to facilitate the growth and antibody secretion of B lymphocyte hybridomas. In this report, a DNA fragment encoding human IL-11 was transduced into fusion partners (mouse myelomas Ag8.653 and SP2/0, and human lymphoblastoid cell line HF2) mediated by lipofection. The transfected cells selected with G418 secreted IL-11 constitutively over the range of 32.4±10.5units/ml to 76.6±18.4units/ml, which could be inhibited by an IL-11 neutralizing MAb up to 80%. doubled, while that of LCLs displayed a 2.4- or 3.3- fold increase, when fused with the transfected fusion partners, respectively. The derived hybridomas from IL-11 secreting fusion partner secreted 3 or 4 times as many immunoglobulins as that from its ancestor. Our data indicate that IL-11 gene transfected fusion partners are improved cell lines for generation of human B lymphocyte hybridomas, and IL-11 may contribute to the increased fusion frequency and antibody secretion of B lymphocyte hybridomas.

Keywords: IL-11, fusion partner, human monoclonal antibody

DOI: 10.3233/HAB-1999-9101

Journal: Human Antibodies, vol. 9, no. 1, pp. 1-7, 1999