Study of TNF-α, IL-1β and LPS levels in the gingival crevicular fluid of a rat model of diabetes mellitus and periodontitis

Article type: Research Article

Authors: Jiang, Zhu-Ling | Cui, Yu-Qiong | Gao, Rui | Li, Ying | Fu, Zhao-Chen | Zhang, Bin | Guan, Cheng-Chao

Affiliations: Department of Implantology, the 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China | Department of Orthodontics, the 1st Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China | Department of Oral and Maxillofacial Surgery, the 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China

Note: [] Corresponding authors: Bin Zhang and Cheng-Chao Guan, Department of Implantology, the 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang, China. E-mail: zhb19622003@yahoo.com.cn, dentiguan@163.com

Abstract: OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis. METHODS: DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Periodontal destruction was assessed by measuring the alveolar bone loss. Periodontal inflammation was quantified by histopathological grading in H&E stained samples. RESULTS: Higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology were found in group 1 compared with group 2, group 3 and group 4 (p< 0.05). The quantities of TNF-α, IL1-β and LPS, the amount of alveolar bone loss and the severity of the histopathological finding were greater in group 2 than group 3 and group 4 (p< 0.05). Group 3 demonstrated higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology than group 4 (p< 0.05). Statistically significant differences were noted between all of the groups. CONCLUSIONS: These data indicate that DM may lead to enhanced TNF-α, IL1-β and LPS production in the periodontal tissues. The resorption values of alveolar bone and the histological inflammation were more severe in rats with periodontitis and diabetes mellitus than in those with periodontitis alone, diabetes mellitus alone and control rats. Our findings are consistent with the hypothesis that hyperglycemia contributes to the heightened inflammatory response associated with periodontitis.

Keywords: TNF-α, IL-1β, LPS, GCF, diabetes mellitus, periodontitis, alveolar bone loss, histopathology

DOI: 10.3233/DMA-130974

Journal: Disease Markers, vol. 34, no. 5, pp. 295-304, 2013