Affiliations: Unidad Académica de Ciencias Químico
Biológicas, Universidad Autónoma de Guerrero, Avenida
Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo,
Guerrero, México | Grupo de Inmunogenética Funcional (IIO),
Departamento de Biología Molecular y Genómica, CUCS, Universidad
de Guadalajara, Juan Díaz Covarrubias y Salvador Quevedo y Zubieta,
Guadalajara, Jalisco, México
Note: [] Corresponding author: Isela Parra-Rojas, PhD, Unidad
Académica de Ciencias Químico Biológicas, Universidad
Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad
Universitaria, Chilpancingo, Guerrero, 39090, México. Tel./Fax: +52 747
472 5503; E-mail: iprojas@yahoo.com
Abstract: Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and is
associated with obesity, dyslipidemias, hypertension (HTN) and type 2 diabetes
mellitus (T2DM). LPL gene polymorphisms can be related with the development of
cardiovascular risk factors. The present study was conducted to analyze the
relationship of the HindIII and S447X polymorphisms in LPL gene with
cardiovascular risk factors in Mexican families. The study population comprised
ninety members of 30 Mexican families, in which an index case had obesity, were
included in the study. We evaluated the body composition by bioelectrical
impedance. Peripheral blood samples were collected to determine biochemical
parameters. Screening for both polymorphisms was made by PCR-RFLPs. In the
parents, both polymorphisms were in Hardy-Weinberg's equilibrium. We found that
the genotype T/T of HindIII was associated with diastolic blood pressure ⩾
85 mmHg (OR=1.1; p=0.011), whereas the genotype C/C of S447X was associated
with systolic blood pressure ⩾ 130 mmHg (OR=1.2; p<0.001), diastolic
blood pressure ⩾ 85 mmHg (OR = 1.3; p< 0.001), T2DM (OR=1.3; p<
0.001) and with increase of total cholesterol (β =23.6 mg/mL; p=0.03).
These data suggest that the HindIII and S447X LPL gene polymorphisms can confer
susceptibility for the development of hypertension and T2DM in Mexican
families.
Keywords: Lipoprotein lipase, polymorphisms, obesity, hypertension, type 2 diabetes
