Circulating levels of high molecular weight (HMW) adiponectin and
total adiponectin in relation to fat distribution, oxidative stress and
inflammation in Asian Indians
Affiliations: Madras Diabetes Research Foundation and Dr.\ Mohan's
Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable
Diseases Prevention and Control, International Diabetes Federation Centre for
Education, Gopalapuram, Chennai, India | Laboratory of Molecular Immunology, Anna University K.
B. Chandrashekar Research Centre, Chennai, India
Note: [] Corresponding author: V. Mohan, Madras Diabetes Research Foundation and Dr. Mohan's Diabetes
Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases
Prevention, Control and IDF Centre for Education 4, Conran Smith Road,
Gopalapuram, Chennai, 600086, India. Tel.: +91 44 4396 8888; Fax: +91 44 2835
0935; E-mail: drmohans@diabetes.ind.in
Abstract: Aim: To look at the association of total and high molecular weight
(HMW) adiponectin with markers of fat distribution, oxidative stress and
inflammation in Asian Indians. Methods: A total of 120 subjects were chosen randomly from Chennai
Urban Rural Epidemiological Study. Fasting HMW adiponectin levels, TNF-alpha
and oxidized LDL were measured using ELISA. High sensitivity C reactive protein
(hsCRP) was measured by a high sensitive nephelometric assay. Lipid
peroxidation was measured by Tbars assay and protein carbonyl content was
assessed by DNPH assay. Visceral and subcutaneous fat areas were assessed by
computed tomography (CT) scan. Results: When stratified based on the tertiles of visceral fat, the
levels of total (p=0.03) and HMW adiponectin (p=0.007) were highest in the
first tertile followed by tertiles 2 and 3 whereas in tertiles of subcutaneous
fat, there was no such trend. With increasing tertiles of Tbars, the levels of
total (p=0.03) and HMW adiponectin decreased (p=0.002). The levels of HMW
(p< 0.001) but not total adiponectin was also found to decrease with
increasing tertiles of Protein carbonyl content. The levels of Total (p=0.02)
and HMW adiponectin (p=0.004) were highest in the first tertile of oxidized LDL
followed by tertile 2 and tertile 3. With increasing tertiles of TNF-alpha
total (p=0.01) and HMW adiponectin (p=0.004) was found to decrease. With
increasing tertiles of hs-CRP, Total (p=0.005) and HMWadiponectin (p=0.007)was
found to decrease. Conclusion: Oxidative stress markers, visceral but not subcutaneous
fat and inflammation are associated with total and HMW adiponectin levles in
Asian Indians.
Keywords: Metabolic syndrome, obesity, type 2 diabetes, Asian Indians, adipocytokines
