Affiliations: Department of Pathological Physiology, Faculty of
Medicine, Masaryk University, Brno, Czech Republic | 1st. Department of Internal Medicine/Cardioangiology,
ICRC, St. Anna Hospital, Masaryk University, Brno, Czech Republic
Note: [] Corresponding author: Prof. Anna Vašků, Department
of Pathological Physiology, Faculty of Medicine, Masaryk University Brno,
University Campus Bohunice, A18, Kamenice 5, 62500 Brno, Czech Republic. Tel.:
+420 549 498 299; Fax: +420 549 494 340; E-mail: avasku@med.muni.cz
Abstract: Atherosclerosis as a main etiopathogenetic source for coronary
artery disease (CAD) development is intimately related to dynamic changes in
the extracellular matrix (ECM). Elevated levels of MMP-13 have been observed in
human atherosclerotic plaques which could also involve variability in MMP-13
gene. The aim of the study was to associate rs640198 polymorphism with CAD
and/or with its severity. The study comprised 1071 consecutive patients with suspected or
known coronary artery disease (CAD), confirmed by coronary angiography.
Genotyping for the rs640198 polymorphism in MMP-13 gene was performed using
Taqman® assay. The TT and TG genotypes of rs640198 polymorphism in MMP-13
gene confer the significantly increased risk of triple vessel disease compared
to patients without atherosclerotic lesions in coronary arteries (odds
ratio=1.64, Pcorr=0.05). Furthermore, an increased risk of having 5 and more
stenoses (odds ratio=1.90, Pcorr=0.004) was observed in TT and TG carriers
(sensitivity of 0.613 and a specificity of 0.544; power of the test is
0.87). The T allele of MMP-13 intron polymorphism rs640198 is associated
with the severity of coronary artery disease, represented by the number of
affected arteries as well as by the number of stenoses confirmed by
coronarography.
