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Article type: Research Article
Authors: Molina-Pinelo, Sonia | Suárez, Rocío | Pastor, María Dolores | Nogal, Ana | Márquez-Martín, Eduardo | Martín-Juan, José | Carnero, Amancio; | Paz-Ares, Luis
Affiliations: Molecular Oncology and New Therapies Group, Department of Medical Oncology, University Hospital Virgen del Rocío, Instituto de Biomedicina de Sevilla, Seville, Spain | Respiratory Disease Medical and Chirurgical Unit, University Hospital Virgen del Rocío, Seville, Spain | Molecular Biology of Cancer Group, Instituto de Biomedicina de Sevilla, Seville, Spain | Consejo Superior de Investigaciones Científicas, Spain
Note: [] Corresponding author: Luis Paz-Ares, MD, PhD, Service of Medical Oncology Hospital Universitario Virgen del Rocío, Manuel Siurot s/n. 41013 Seville, Spain. Tel.: +34 955 013414; Fax: +34 955 013 292; E-mail: luis.pazares.sspa@juntadeandalucia.es
Abstract: The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome of respiratory pathological processes. The measurement of miRNA expression through less invasive techniques such as plasma and serum have been suggested to analysis of several lung malignancies including lung cancer. These studies are assuming a common deregulated miRNA expression both in blood and lung tissue. The present study aimed to obtain miRNA representative signatures both in plasma and bronchoalveolar cell fraction that could serve as biomarker in respiratory diseases. Ten patients were evaluated to assess the expression levels of 381 miRNAs. We found that around 50% miRNAs were no detected in both plasma and bronchoalveolar cell fraction and only 20% of miRNAs showed similar expression in both samples. These results show a lack of association of miRNA signatures between plasma and bronchoalveolar cytology in the same patient. The profiles are not comparable; however, there is a similarity in the relative expression in a very small subset of miRNAs (miR-17, miR-19b, miR-195 and miR-20b) between both biological samples in all patients. This finding supports that the miRNAs profiles obtained from different biological samples have to be carefully validated to link with respiratory diseases.
Keywords: miRNA profile, RT-qPCR, plasma and bronchoalveolar fluid cytology, respiratory disease
DOI: 10.3233/DMA-2011-0882
Journal: Disease Markers, vol. 32, no. 4, pp. 221-230, 2012
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