Affiliations: Department of Toxicogenomics, National Institute of
Public Health, Prague, Czech Republic | First Faculty of Medicine, Charles University in
Prague, Czech Republic | Department of Transplantation Surgery, Institute of
Clinical and Experimental Medicine, Prague, Czech Republic | Department of Clinical and Transplantation Pathology,
Institute of Clinical and Experimental Medicine, Prague, Czech Republic
Note: [] Address for correspondence: Pavel Soucek, PhD., Toxicogenomics
Unit, National Institute of Public Health, Srobarova 48, Prague 10, 100 42
Czech Republic. Tel.: +420 267082711; Fax: +420 267311236; E-mail:
psoucek@szu.cz
Abstract: Histologically verified pairs (n=10) of
pancreatic tumors and non-neoplastic tissues were used for quantitative
real-time PCR and the stability of 24 reference genes was analyzed with geNorm
and NormFinder software. Raw C_{q} values correlated with
the degree of RNA degradation. This correlation was abolished by normalization
to C_{q} of 18S endogenous control gene. Both geNorm and
NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the
same most stable genes. We have thus identified suitable reference genes for
future expression studies in pancreatic carcinoma. Normalization method
reducing the effects of RNA degradation on the quality of results was also developed.
