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Article type: Research Article
Authors: Tomar, Anil Kumar | Sooch, Balwinder Singh | Raj, Isha | Singh, Sarman | Singh, Tej P. | Yadav, Savita
Affiliations: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India | Department of Biotechnology, Punjabi University, Patiala, India | School of Life Sciences, Jawaharlal Nehru University, New Delhi, India | Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India
Note: [] Corresponding author: Savita Yadav, Additional Professor, Department of Biophysics, All India Institute of Medical sciences, New Delhi 110029, India. E-mail: savita11@gmail.com
Abstract: Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma.
Keywords: Azoospermia, glycoproteins, lectin affinity chromatography, male infertility, oligospermia
DOI: 10.3233/DMA-2011-0853
Journal: Disease Markers, vol. 31, no. 6, pp. 379-386, 2011
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