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Article type: Research Article
Authors: Fernandez, Sonia | French, Martyn A.; | Price, Patricia;
Affiliations: School of Pathology and Laboratory Medicine, University of Western Australia and PathWest Laboratory Medicine, Perth, Australia | Department of Clinical Immunology, Royal Perth Hospital and PathWest Laboratory Medicine, Perth, Australia
Note: [] Corresponding author: Dr. Sonia Fernandez, School of Pathology and Laboratory Medicine, University of Western Australia, Level 2, MRF Building, Rear 50 Murray St, Perth WA 6001, Australia. Tel.: +61 8 92240223; Fax: +61 8 92240204; E-mail: sonia.fernandez@uwa.edu.au
Abstract: HIV-infected individuals responding to antiretroviral therapy (ART) after severe CD4^{+} T-cell depletion may retain low responses to recall antigens [eg: cytomegalovirus (CMV)] and altered expression of T-cell co-stimulatory molecules consistent with immunosenescence. We investigated the capacity of phenotypically senescent cells to generate cytokines in HIV patients receiving long-term ART (n=18) and in healthy controls (n=10). Memory T-cells were assessed by interferon (IFN)-γ ELISpot assay and flow cytometrically via IFN-γ or IL-2. Proportions of CD57^{bright}CD28^{null} CD4^{+} T-cells correlated with IFN-γ responses to CMV (p=0.009) and anti-CD3 (p=0.002) in HIV patients only. Proportions of CD57^{bright}CD28^{null} CD8^{+} T-cells and CD8^{+} T-cell IFN-γ responses to CMV peptides correlated in controls but not HIV patients. IL-2 was predominantly produced by CD28^{+}T-cells from all donors, whereas IFN-γ was mostly produced by CD57^{+} T-cells. The findings provide evidence of an accumulation of immunosenescent T-cells able to make IFN-γ. This may influence the pathogenesis of secondary viral infections in HIV patients receiving ART.
Keywords: CD57, HIV, immune activation, immunosenescence
DOI: 10.3233/DMA-2011-0847
Journal: Disease Markers, vol. 31, no. 6, pp. 337-342, 2011
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