Affiliations: Endocrinology and Metabolism Research Center, Tehran
University of Medical Sciences, Tehran, Iran | Department of Medical Genetics, Tehran University of
Medical Sciences, Tehran, Iran
Note: [] Corresponding author: Javad Tavakkoly-Bazzaz, MD, PhD.,
Department of Medical Genetics, Tehran University of Medical Sciences, Poursina
St., Tehran, 14176, Iran. Tel./Fax: +98 21 88953005; E-mail:
tavakkolybazzazj@tums.ac.ir
Abstract: Objective: Klotho has an important role in development of coronary
artery (CAD) disease. A functional variant of klotho gene (kl-vs) has been
found as an independent risk factor for early-onset occult coronary artery
disease (CAD) in previous studies. The Frequency of this variant was not known
in Iranian population. We have examined the allele frequency of the kl-vs
variant in a case-control study in an Iranian population. Methods and results: Genotyping for kl-vs variant was carried out in
N=107 individuals including N=54 cases and N=53 control who all underwent
coronary angiogram for CAD evaluation. Patients with >50% stenosis in
vessels considered as case groups (or CAD^{+}) and patients
with normal vessels (or CAD^{-}) as controls. The frequency
of kl-vs variant was determined in these patients using PCR-RFLP technique.
None of the individual was carrying the kl-vs mutation in our samples. The
frequency of kl-vs mutation was significantly different from previous studies
in different populations. Conclusion: The kl-vs variant seems to be scare found in the Iranian
population in comparison to other populations reported previously. Klotho gene
might be a candidate gene of atherosclerosis in some populations but not in
Iranian population. Further studies are required to examine the frequency of
kl-vs variant in other populations from the Middle East.
