Affiliations: Department of Neurology, Sanjay Gandhi Post Graduate
Institute of Medical Sciences, Lucknow, India | Department of Radiodiagnosis, Sanjay Gandhi Post
Graduate Institute of Medical Sciences, Lucknow, India | Department of Medical Genetics, Sanjay Gandhi Post
Graduate Institute of Medical Sciences, Lucknow, India
Note: [] Corresponding author: J. Kalita, Additional Professor,
Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical
Sciences, Raebareily Road, Lucknow- 226014, India. Tel.: +91 522 2668811; Fax:
+91 522 2668017; E-mail: jayanteek@yahoo.com, jkalita@sgpgi.ac.in
Abstract: In ischemic stroke, extracranial MR angiography (ECMRA) is more
frequently abnormal in Caucasians and intracranial (ICMRA) in Asians which may
have a genetic basis. We report phosphodiesterase (PDE4D) gene polymorphism and
its correlation with MRA findings in patients with ischemic stroke. Consecutive patients with MRI proven ischemic stroke undergoing MRA
were included in this study. The severity of atherosclerotic stenosis on MRA
was categorized into moderate 50%–80%, severe 80%–99%, and total
occlusion 100% using NASCET criteria. The polymorphism in SNP 32, SNP 83 and
SNP 87 of PDE4D gene was analyzed by PCR both in the patients and in 188
controls. Among the 148 patients, MRA was abnormal in 77% patients; ECMRA in
53.8%, ICMRA in 66% and both were abnormal in 42% patients. The frequency of CC
genotype of PDE4D83 was significantly higher in the patients with ischemic
stroke compared to controls (OR 3.38, 95% CI 1.61–7.11, P= 0.001). The
frequency of TT genotype of PDE4D87 was significantly higher ICMRA
abnormalities (20%) compared to normal ICMRA (2%). The genotype and allele
frequency of PDE4D83 and PDE4D32 were not significantly related to MRA
abnormalities. The role of PDE4D87 in atherosclerosis needs confirmation in
larger studies.
