Affiliations: Department of Obstetrics and Gynecology, Jessenius
Faculty of Medicine, Comenius University, Martin, Slovakia | Department of Hematology and Transfusiology, Jessenius
faculty of Medicine, Comenius University, Martin, Slovakia
Note: [] Corresponding author: Kristina Biskupska Bodova, Department of
Obstetrics and Gynecology, Jessenius Faculty of Medicine in Martin, Comenius
University in Bratislava, Kollarova 2, 036 59 Martin, Slovakia. Tel.: +386 421
905 783 076; Fax: +386 421 4203 413 41 85; E-mail:
kristina.biskupska@post.sk
Abstract: Objective: The purpose of this study was to examine plasma levels of
fibronectin and plasminogen inhibitor type 1 (PAI-1), and alterations in
uterine artery (UtA) waveforms throughout normotensive and preeclamptic
pregnancies and to analyze its predictive value for the detection of
preeclampsia within the second trimester of pregnancy. Material and methods: Blood samples were collected from 102 healthy,
nulliparous women between the 24th and 26th gestational week. Preeclampsia
developed in 13 patients; 89 normotensive control subjects were matched from
the same cohort. Plasma samples were assayed for fibronectin and PAI-1 by
enzyme-linked immunosorbent assay. Color pulsed Doppler examinations of UtA
were performed after blood sampling. Trends were compared between two groups.
Results: Maternal plasma fibronectin and PAI-1 levels and average
PI, RI and S/D ratios of patients with preeclampsia were significantly higher
(p< 0.05). The best cut-off values for predicting preeclampsia of
fibronectin, PAI-1, PI, RI, S/D ratio based on ROC curve analysis were 290
mg/ml, 77.3 ng/ml, 1,0615, 0.605 and 2,59 respectively. The areas under the
curve equal to 0.705, 0.753, 0.689, 0.695 and 0.699 for fibronectin, PAI-1 and
uterine artery Doppler PI, RI, S/D ratio were determined for the prediction of
preeclampsia. Conclusions: Fibronectin, PAI-1 and UtA Doppler are potentially
useful predictors of preeclampsia. Maternal plasma PAI-1 combinated with
fibronectin had the highest predictive values in our study.
