Tumor necrosis factor-alpha gene promoter −308G/A and −238G/A polymorphisms in Mexican patients with type 2 diabetes mellitus

Article type: Research Article

Authors: Guzmán-Flores, Juan Manuel | Muñoz-Valle, José Francisco | Sánchez-Corona, José | Cobián, José G. | Medina-Carrillo, Leopoldo | G. García-Zapién, Alejandra | Cruz-Quevedo, Edhit G. | Flores-Martínez, Silvia Esperanza

Affiliations: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México | Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, México | Hospital General de Zona No. 1, Instituto Mexicano del Seguro Social, Colima, Colima, México | Coordinación Delegacional de Investigación en Salud, Instituto Mexicano del Seguro Social, Tepic, Nayarit, México

Note: [] Corresponding author: Silvia Esperanza Flores-Martínez, Ph.D., División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Sierra Mojada 800, Col. Independencia, C.P. 44340, Guadalajara, Jalisco, México. Tel.: +52 3336 189410; Fax: +52 3336 181756; E-mail: sefloresm@yahoo.com and silvia.floresma@imss.gob.mx

Abstract: The {association} between some Tumor necrosis factor-alpha (TNF-α) promoter polymorphisms and Type 2 diabetes mellitus (T2DM) remains controversial. Ethnic differences may play a role in these conflicting results. The aim of this study was to investigate the association between −308G/A and −238G/A polymorphisms located in the promoter region of the TNF-α gene and T2DM in Mexican mestizo patients. Nine hundred four individuals (259 patients with T2DM and 645~controls) were genotyped for the −308G/A and −238G/A polymorphisms by PCR--RFLP. We found that the −238A allele increased the risk of developing T2DM in Mexican patients (OR=1.57, 95% CI: 1.07–2.29; p=0.018). Moreover, we found that the frequency of the GA haplotype (created by the −308G and −238A alleles) was significantly increased in patients with T2DM when compared with controls (OR =1.56, 95% CI: 1.05–2.31; p=0.026). Our results suggest that the −238G/A polymorphism and a specific haplotype (GA) are genetic risk factors for the development of T2DM in Mexican population.

Keywords: Haplotype, Mexican patients, polymorphism, TNF-alpha, Type 2 diabetes mellitus

DOI: 10.3233/DMA-2011-0759

Journal: Disease Markers, vol. 30, no. 1, pp. 19-24, 2011