Affiliations: Department of Biochemistry, University of Allahabad,
Allahabad, India
Note: [] Corresponding author: Dr. Syed Ibrahim Rizvi, Department of
Biochemistry, University of Allahabad, Allahabad, 211002, India. Tel.: +91
9415305910; E-mail: sirizvi@gmail.com
Abstract: Previous studies have indicated that the main molecular
characteristic of aging is the progressive accumulation of oxidative damages in
cellular macromolecules. Proteins are one of the main molecular targets of
age-related oxidative stress, which have been observed during aging process in
cellular systems.. Reactive oxygen species (ROS) can lead to oxidation of amino
acid side chains, formation of protein-protein cross-linkages, and oxidation of
the peptide backbones. In the present study, we report the age-dependent
oxidative alterations in biomarkers of plasma protein oxidation: protein
carbonyls (PCO), advanced oxidation protein products (AOPPs) and plasma total
thiol groups (T-SH) in the Indian population and also correlate these
parameters with total plasma antioxidant potential. We show an age dependent
decrease in T-SH levels and increase in PCO and AOPPs level. The alterations in
the levels of these parameters correlated significantly with the total
antioxidant capacity of the plasma. The levels of oxidized proteins in plasma
provide an excellent biomarker of oxidative stress due to the relative long
half-life of such oxidized proteins.
Keywords: Human aging, oxidative stress, AOPPs, protein carbonyls, -SH
