Affiliations: First Department of Medicine, University of Debrecen
Medical and Health Science Center, Debrecen, Hungary | 1st Department of Surgery, University of Debrecen
Medical and Health Science Center, Debrecen, Hungary | B.Braun Dialysis Center, Kenézy Gyula Hospital,
Debrecen, Hungary | B.Braun Dialysis Center, Nyíregyháza,
Hungary
Note: [] Corresponding author: Ildikó Seres PhD, First Department
of Medicine, University of Debrecen, Medical and Health Science Center,
Nagyerdei krt. 98. H-4032 Debrecen, Hungary. Tel./Fax: +36 52 442 101; E-mail:
seres@internal.med.unideb.hu
Abstract: Background: Human paraoxonase-1 (PON1) inhibits LDL-oxidation and
atherogenesis, and possesses lactonase activity. Decreased PON1 activity was
found in hemodialyzed and renal transplanted patients. Cystatin C plays a
protective role in atherosclerosis, and is a new, sensitive marker of renal
function. The relationship between these two markers in renal failure has not
been investigated. Aims: The goal of this study was to clarify the relationship
between PON1 activity, cystatin C and homocysteine in chronic renal failure. We
also determined the levels of oxidatively modified LDL (oxLDL) and
thiobarbituric acid reactive substances (TBARS) to characterize lipid
peroxidation. Patients and methods: 74 hemodialized (HD), 171 renal
transplanted patients (TRX), and 110 healthy controls (C) were involved in the
study. PON1 activity and TBARS levels were measured spectrophotometrically.
OxLDL level was determined with sandwich ELISA. Results: There was a negative correlation between PON1
activity and cystatin C level. Homocysteine level correlated negatively with
PON1 activity, and positively with cystatin C level. OxLDL and TBARS levels
were significantly higher in the HD and TRX groups compared to C. Conclusions: Cystatin C may be a good predictive factor not
only for homocysteine levels but for the antioxidant status in patients with
renal failure and renal transplantation.
