Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines

Issue title: Disease related glycosylation changes and biomarker discovery: Challenges and possibilities in an emerging field

Article type: Research Article

Authors: de Leoz, Maria Lorna A. | An, Hyun Joo | Kronewitter, Scott | Kim, Jaehan | Beecroft, Sean | Vinall, Ruth | Miyamoto, Suzanne | de Vere White, Ralph | Lam, Kit S. | Lebrilla, Carlito^;

Affiliations: Department of Chemistry, University of California Davis, Davis, CA, USA | Department of Viticulture and Enology, University of California Davis, Davis, CA, USA | Department of Urology, University of California Davis Medical Center, Sacramento, CA, USA | Division of Hematology and Oncology, University of California Davis Medical Center, Sacramento, CA, USA | Department of Biochemistry, School of Medicine, University of California Davis, Davis, CA, USA

Note: [] Corresponding author. Tel.: +1 530 752 6364; Fax: +1 530 752 8995; E-mail: cblebrilla@ucdavis.edu

Abstract: Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.

Keywords: Biomarker, glycans, mass spectrometry, prostate cancer, serum

Journal: Disease Markers, vol. 25, no. 4-5, pp. 243-258, 2008