Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Galamb, Orsolya; | Györffy, Balázs | Sipos, Ferenc | Spisák, Sándor | Németh, Anna Mária | Miheller, Pál | Tulassay, Zsolt; | Dinya, Elek | Molnár, Béla;
Affiliations: 2nd Department of Medicine, Semmelweis University, Budapest, Hungary | Joint Research Laboratory of the Hungarian Academy of Sciences and the Semmelweis University for Pediatrics and Nephrology, 1st Department of Paediatrics, Semmelweis University Budapest, Hungary | EGIS Pharmaceuticals Ltd. Medical Department, Budapest, Hungary | Hungarian Academy of Science, Molecular Medicine Research Unit, Budapest, Hungary
Note: [] Corresponding author: Orsolya Galamb, 2nd Department of Medicine Semmelweis University, 1088 Budapest, Szentkirályi str. 46, Hungary. Tel.: +36 1 266 09 26; Fax: +361 266 08 16; E-mail: orsg1@yahoo.com
Abstract: Gene expression analysis of colon biopsies using high-density oligonucleotide microarrays can contribute to the understanding of local pathophysiological alterations and to functional classification of adenoma (15 samples), colorectal carcinomas (CRC) (15) and inflammatory bowel diseases (IBD) (14). Total RNA was extracted, amplified and biotinylated from frozen colonic biopsies. Genome-wide gene expression profile was evaluated by HGU133plus2 microarrays and verified by RT-PCR. We applied two independent methods for data normalization and used PAM for feature selection. Leave one-out stepwise discriminant analysis was performed. Top validated genes included collagenIVα1, lipocalin-2, calumenin, aquaporin-8 genes in CRC; CD44, met proto-oncogene, chemokine ligand-12, ADAM-like decysin-1 and ATP-binding casette-A8 genes in adenoma; and lipocalin-2, ubiquitin D and IFITM2 genes in IBD. Best differentiating markers between Ulcerative colitis and Crohn's disease were cyclin-G2; tripartite motif-containing-31; TNFR shedding aminopeptidase regulator-1 and AMICA. The discriminant analysis was able to classify the samples in overall 96.2% using 7 discriminatory genes (indoleamine-pyrrole-2,3-dioxygenase, ectodermal-neural cortex, TIMP3, fucosyltransferase-8, collectin sub-family member 12, carboxypeptidase D, and transglutaminase-2). Using routine biopsy samples we successfully performed whole genomic microarray analysis to identify discriminative signatures. Our results provide further insight into the pathophysiological background of colonic diseases. The results set up data warehouse which can be mined further.
Keywords: Gene expression signature, whole genomic oligonucleotide microarray, colon cancer, adenoma, inflammatory bowel disease
Journal: Disease Markers, vol. 25, no. 1, pp. 1-16, 2008
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl