Affiliations: Department of Biology, Medical Genetics and Ecology,
Kursk State Medical University, Kursk, Russia | Kursk Regional Clinical Hospital, Kursk, Russia | Kursk Emergency Medicine Hospital, Kursk, Russia
Note: [] Corresponding author: Alexey V. Polonikov, MD, PhD., Department
of Biology, Medical Genetics and Ecology, Kursk State Medical University, Karl
Marx Str. 3, 305041 Kursk, Russia. Tel./Fax: +7 4712 58 81 47; E-mail:
polonikov@rambler.ru
Abstract: The present study was designed to test whether common polymorphism
G-50T within the promoter of human CYP2J2 gene is associated with
increased risk of essential hypertension in a Russian population. We studied
576 unrelated subjects, including 295 patients with hypertension and 281
healthy subjects. Genotyping for polymorphism G-50T of the CYP2J2 gene
was performed by polymerase chain reaction and restriction fragment length
polymorphism techniques. The frequency of a −50T variant allele of
CYP2J2 gene was significantly higher in patients with hypertension versus
healthy controls (OR 4.03 95%CI 1.80–9.04 p=0.0004). The association of
a −50GT genotype with hypertension remained significant after adjustment for
age, gender and family history of hypertension by multivariate logistic
regression (OR 4.78 95%CI 1.87–12.27 p=0.001). It has been found that OR
for −50GT genotype × gender interaction (OR 4.48 95%CI 1.93–10.39
p=0.00048) was slightly higher than OR for −50GT genotype (OR 4.43
95%CI 1.91–10.29 p=0.00052), suggesting a weak effect of gender on the
risk of hypertension in the heterozygous carriers of −50GT genotype. A family
history of hypertension has no effect on the association between a −50GT
genotype and hypertension. In present study we demonstrate for the first time
that a CYP2J2*7 allele of the CYP2J2 gene is clearly associated
with an increased risk of essential hypertension. Furthermore, this study
highlights the importance of P-450 epoxygenase pathway of arachidonic acid
metabolism in the pathogenesis of hypertensive disease.
