Affiliations: Department of Biochemistry, All India Institute of
Medical Sciences, New Delhi-110029, India | Department of Cardiology, All India Institute of
Medical Sciences, New Delhi-110029, India | Department of Cardiac Biochemistry, All India
Institute of Medical Sciences, New Delhi-110029, India
Note: [] Corresponding author: Dr. Nibhriti Das, Professor, Department of
Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New
Delhi-110029, India. Tel.: +91 1 126593467; Fax: +91 1 126862663; E-mail:
nibhriti@hotmail.com
Abstract: Apolipoprotein A-I (APOA1 gene, apoA-I protein) is the major protein
for plasma high density lipoprotein (HDL). The relationship of APOA1-75G/A
polymorphism with lipid profile and coronary artery disease (CAD) is unclear.
Out of 370 individuals initially recruited, 164 angiographically proven CAD
patients (⩾ 70% stenosis) and 36 individuals with normal coronaries or
insignificant CAD (NCAD, ⩽ 50% stenosis) from Delhi and adjoining areas
were selected for analysis based on the set criteria. Polymorphism was
determined by PCR followed by MspI restriction digestion. Lipid profile was
estimated by enzymatic kit and apoA-I levels by immunoturbidimetry. A highly
significant increasing trend in 'A' allele frequency was observed with the rise
in severity of CAD: NCAD (0.097) < SVD (single vessel disease) (0.117) <
DVD (double vessel disease) (0.223) < TVD (triple vessel disease) (0.291).
In comparison to GG individuals, the OR of 'A' allele carriers to develop SVD,
DVD, TVD was 1.3, 2.8 and 4.2 respectively (p_{trend} =
0.007). Analysis of intergenotypic variations in the lipid profile revealed
significantly lower levels of HDL and apoA-I among 'A' allele carriers as
compared to GG (patients). Our study, first of its kind from India, suggests
that 'A' allele may contribute to severity of CAD and low levels of HDL &
apoA-I. However, an in depth study with a larger set of sample is
necessary.
Keywords: APOA1, apolipoprotein, CAD, polymorphism, India
