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Issue title: Functional Imaging of Early Markers of Disease. Part 2
Article type: Research Article
Authors: Costa, Kevin D.
Affiliations: Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
Note: [] Address for correspondence: Kevin D. Costa, Ph.D., Columbia University, Department of Biomedical Engineering, 351 Engineering Terrace, Mail Code 8904, 1210 Amsterdam Avenue, New York, NY 10027, USA. Tel.: +1 212 854 9163; Fax: +1 212 854 8725; E-mail: kdc17@columbia.edu
Abstract: The atomic force microscope (AFM) is emerging as a powerful tool in cell biology. Originally developed for high-resolution imaging purposes, the AFM also has unique capabilities as a nano-indenter to probe the dynamic viscoelastic material properties of living cells in culture. In particular, AFM elastography combines imaging and indentation modalities to map the spatial distribution of cell mechanical properties, which in turn reflect the structure and function of the underlying cytoskeleton. Such measurements have contributed to our understanding of cell mechanics and cell biology and appear to be sensitive to the presence of disease in individual cells. This chapter provides a background on the principles and practice of AFM elastography and reviews the literature comparing cell mechanics in normal and diseased states, making a case for the use of such measurements as disease markers. Emphasis is placed on the need for more comprehensive and detailed quantification of cell biomechanical properties beyond the current standard methods of analysis. A number of technical and practical hurdles have yet to be overcome before the method can be of clinical use. However, the future holds great promise for AFM elastography of living cells to provide novel biomechanical markers that will enhance the detection, diagnosis, and treatment of disease.
Keywords: cell biomechanics, viscoelasticity, cytoskeleton, indentation, stiffness, disease markers
Journal: Disease Markers, vol. 19, no. 2-3, pp. 139-154, 2004
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