Affiliations: Department of Pediatrics, University of Michigan
Medical Center, Ann Arbor, MI 48109, USA | Department of Surgery, University of Michigan Medical
Center, Ann Arbor, MI 48109, USA
Note: [] University of Michigan Medical Center, 1150 W. Medical Center
Drive, A520 Medical Science Research Building I, Ann Arbor, MI 48109-0656, USA.
Tel.: +1 734 763 9311; Fax: +1 734 647 8148; E-mail: shanash@umich.edu
Abstract: We have developed a comprehensive approach to the identification of
protein markers in lung cancer that includes profiling of tumor tissue and cell
lines as well as the analysis of serum for autoantibodies to lung tumor
antigens. A large number of proteins that are differentially expressed in the
major subtypes of lung cancer have been identified by mass spectrometry. A
database of protein expression in lung cancer and other types of cancer has
been constructed that integrates two-dimensional gel profiles, mass
spectrometry data, quantitative protein data and gene expression data at the
RNA level, that serves as a resource for biomarker identification. Analysis of
the serological response in lung cancer has led to the identification of novel
markers detectable in serum of lung cancer patients at the time of diagnosis.
The proteomic approach is likely to yield novel classification schemes and
novel markers for early diagnosis of lung cancer.
